INCREASE IN SPERMINE CONTENT COORDINATED WITH SIDEROPHORE PRODUCTION IN PARACOCCUS-DENITRIFICANS
INCREASE IN SPERMINE CONTENT COORDINATED WITH SIDEROPHORE PRODUCTION IN PARACOCCUS-DENITRIFICANS
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DOI：
10.1128/jb.173.7.2238-2243.1991
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发表时间：
1991-04-01
影响因子：
3.2
通讯作者：
WEIMAR, WR
中科院分区：
文献类型：
--
作者：
BERGERON, RJ;WEIMAR, WR
精胺存在于在补充有50 μ M铁（III）的琥珀酸铵最低盐培养基中需氧培养的副球菌中，其含量相对较低。 然而，在缺铁培养物[含有0.5 μ M铁（III）的最低盐培养基]中，精胺含量增加了一个数量级，与众所周知的缺铁反应相协调，例如，铁载体合成和铁载体排泄的去抑制。 当铁剥夺文化表现出高精胺含量和强大的铁载体生产restened到新鲜的最低盐培养基含有50 μ M铁[III]，铁载体生产和精胺含量迅速下降。 补铁后5小时，精胺低于检测限。 这些结果表明，精胺的响应中的特定作用的P.acerificans低铁胁迫。
Spermine is present in relatively low amounts in Paracoccus denitrificans cultured aerobically in an ammonium succinate minimal salts medium supplemented with 50-mu-M iron(III). However, in iron-deprived cultures [minimal salts medium containing 0.5-mu-M iron(III)], spermine content increases by an order of magnitude in coordination with the well-known responses to iron deprivation, e.g., derepression of siderophore synthesis and siderophore excretion. When iron-deprived cultures exhibiting both high spermine content and strong siderophore production are reseeded into fresh minimal salts medium containing 50-mu-M iron[III], both siderophore production and spermine content fall rapidly. Five hours after iron supplementation, spermine is below limits of detection. These results suggest a specific role for spermine in the response of P. denitrificans to low-iron stress.