Factors that impact the nacuum ultraviolet photofragmentation of peptide ions
Factors that impact the nacuum ultraviolet photofragmentation of peptide ions
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DOI：
10.1016/j.jasms.2007.04.015
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发表时间：
2007-08-01
影响因子：
3.2
通讯作者：
Reilly, James P.
中科院分区：
文献类型：
--
作者：
Thompson, Matthew S.;Cui, Weidong;Reilly, James P.
几个研究小组已经研究了多肽离子在紫外光下的光解作用。在157 nm和193 nm激发下有显著差异。最近的研究表明，质谱分析仪也可以影响观察到的光碎片分布。比较实验使用不同的肽，波长，和质量分析仪是不必要的复杂。在本工作中，用串联tof和线性离子阱质谱仪分析了几种多肽的157和193运行光解。结果表明，光解波长和质谱分析仪对离子的分布都有影响。这两种波长在离子阱中产生相似的光谱，但在串联tof仪器中产生截然不同的结果。
Several groups have investigated the photodissociation of peptide ions with ultraviolet light. Significant differences have been reported with 157 and 193 nm excitation. Recent studies have shown that the mass analyzer can also influence the observed photofragment distribution. Comparison of experiments using different peptides, wavelengths, and mass analyzers is undesirably complicated. In the present work, several peptides are analyzed with both 157 and 193 run photodissociation in tandem-TOF and linear ion trap mass spectrometers. The results indicate that the fragment ion distribution can be influenced by both the photodissociation wavelength and the mass analyzer. The two wavelengths generate similar spectra in an ion trap but quite different results in a tandem-TOF instrument.