The chemical biomarkers C2C, Coll2-1, and Coll2-1NO2 provide complementary information on type II collagen catabolism in healthy and osteoarthritic mice
The chemical biomarkers C2C, Coll2-1, and Coll2-1NO2 provide complementary information on type II collagen catabolism in healthy and osteoarthritic mice
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DOI：
10.1002/art.22875
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发表时间：
2007-10-01
影响因子：
--
通讯作者：
Henrotin, Y.
中科院分区：
文献类型：
--
作者：
Ameye, L. G.;Deberg, M.;Henrotin, Y.
目标。与野生型（WT）小鼠相比，biglycan/纤维调蛋白双缺陷小鼠发生严重的膝关节骨关节炎。我们进行了这项研究，以比较两种基因型中的11型胶原分解代谢，并比较3种胶原降解生物标志物（C2C[也称为Col23/4C（长单核苷酸）]以及肽Coll2-1及其硝化形式Coll2-1NO(2)）在体内评估胶原分解代谢的有效性。在15只WT小鼠和15只biglycan/纤维调节蛋白双缺陷小鼠中，我们测定了66天和141天时血清C2C水平，并测定了49、81、95和141天时血清Coll2-1和Coll2-1NO(2)水平。采用免疫组织化学方法检测膝关节切片中生物标志物的表达。在所有时间点，高聚糖/纤维调节蛋白双缺陷小鼠C2C和Coll2-1的平均浓度均较高。对于C2C和Coll2-1， biglycan/ fibromodulin双缺陷小鼠的血清浓度与WT小鼠的血清浓度之比（双缺陷：WT之比）随时间不变，分别近似于1.63和1.15。相比之下，col2 - 1no(2)的双缺陷：WT比有所不同，根据年龄的不同，为bb0.1或bb0.1
Objective. Compared with wild-type (WT) mice, biglycan/fibromodulin double-deficient mice develop severe knee osteoarthritis. We undertook this study to compare type 11 collagen catabolism in the 2 genotypes and to compare the usefulness of 3 biomarkers of collagen degradation (C2C [also known as Col23/4C(long mono)] as well as the peptide Coll2-1 and its nitrated form, Coll2-1NO(2)) for evaluating collagen catabolism in vivo.Methods. In 15 WT mice and 15 biglycan/ fibromodulin double-deficient mice, we determined serum levels of C2C at ages 66 and 141 days, and we determined serum levels of Coll2-1 and Coll2-1NO(2) at ages 49, 81, 95, and 141 days. Expression of the biomarkers in knee sections was examined using immunohistochemistry.Results. The mean concentrations of C2C and Coll2-1 were higher in biglycan/fibromodulin double-deficient mice at all time points. For C2C and Coll2-1, the ratio of the serum concentration in biglycan/ fibromodulin double-deficient mice to that in WT mice (the double-deficient:WT ratio) was constant over time and was similar to 1.63 and similar to 1.15, respectively. In contrast, the double-deficient:WT ratio for Coll2-1NO(2) varied and, depending on age, was >1 or