Behavioral effects of local microinfusion of pituitary adenylate cyclase activating polypeptide (PACAP) into the paraventricular nucleus of the hypothalamus (PVN)
Behavioral effects of local microinfusion of pituitary adenylate cyclase activating polypeptide (PACAP) into the paraventricular nucleus of the hypothalamus (PVN)
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DOI：
10.1016/j.regpep.2004.12.023
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发表时间：
2005-05-15
影响因子：
--
通讯作者：
Légrádi, G
中科院分区：
文献类型：
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作者：
Norrholm, SD;Das, M;Légrádi, G
腺苷酸环化酶激活多肽（PACAP）参与了多种自主神经和神经内分泌功能的调节。例如，在下丘脑室旁核（PVN），PACAP免疫反应纤维密集地支配内侧小细胞区含促肾上腺皮质激素释放激素（CRH）的神经元，这表明PACAP起着介导应激反应的作用。因此，我们研究了PVN内PACAP注射（25 pmol）与轻度应激相结合的行为效应。PVN内微量注射PACAP或人工脑脊液（aCSF）（0.25 1），然后将动物束缚或置于其饲养笼中5分钟。（总行驶距离）和行为评分在熟悉的开放视野中观察10分钟（洗脸、身体梳理、湿狗摇动和饲养）。在接受aCSF的动物中，限制组和不限制组之间没有行为差异。在整个10分钟的观察期内，接受PACAP的动物，无论是否受约束，都表现出洗脸和身体梳理增加，运动活动和直立减少。在PACAP注射的动物中，在开放视野的前2分钟期间，与未受限制的动物相比，受限制的动物显示出增加的身体梳理，这表明肽注射和应激物的作用的总和。所观察到的理毛升高与先前的研究报告类似的增加后，电，NMDA，CRH，或应激诱导的PVN激活。因此，在PVN水平，PACAP可能作为一种兴奋性神经肽和增强行为反应的压力。(c)2004 Elsevier B.V保留所有权利。
Pituitary adenylate cyclase activating polypeptide (PACAP) has been implicated in the regulation of several autonomic and neuroendocrine functions. In the hypothalamic paraventricular nucleus (PVN), for example, PACAP-immunoreactive fibers densely innervate corticotropin-releasing hormone (CRH)-containing neurons in the medial parvocellular region, suggesting that PACAP acts to mediate stress responses. Therefore, we examined the behavioral effects of an intra-PVN PACAP injection (25 pmol) in combination with a mild stressor. PACAP or artificial cerebrospinal fluid (aCSF) was microinjected into the PVN (0.25 1) and then animals were restrained or placed in their home cage for 5 min. Exploratory activity (total distance traveled) and scored behaviors (face washing, body grooming, wet dog shakes, and rearing) were observed in a familiar open field for 10 min. In animals receiving aCSF, there were no behavioral differences between restrained and unrestrained groups. For the entire 10-min observation period, animals receiving PACAP, whether restrained or not, displayed elevated face washing and body grooming with decreased locomotor activity and rearing. Among PACAP-injected animals, restrained animals displayed increased body grooming compared to unrestrained animals during the first 2 min in the open field suggesting a summation of the effects of peptide injection and stressor. The observed elevation in grooming is consistent with previous studies reporting similar increases following electrical-, NMDA-, CRH-, or stressor-induced activation of the PVN. Thus, at the level of the PVN, PACAP may act as an excitatory neuropeptide and augment behavioral responses to stressors. (c) 2004 Elsevier B.V All rights reserved.