An automated and fully validated LC-MS/MS procedure for the simultaneous determination of 11 opioids used in palliative care, with 5 of their metabolites
An automated and fully validated LC-MS/MS procedure for the simultaneous determination of 11 opioids used in palliative care, with 5 of their metabolites
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DOI：
10.1002/jms.1021
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发表时间：
2006-05-01
影响因子：
2.3
通讯作者：
Madea, B.
中科院分区：
文献类型：
--
作者：
Musshoff, F.;Trafkowski, J.;Madea, B.
建立了电喷雾电离串联质谱联用（LC-ESI-MS/MS）定量测定血浆中阿片类药物丁丙诺啡、可待因、芬太尼、氢吗啡酮、美沙酮、吗啡、羟考酮、吡拉西米、tilidine和曲马多及其代谢物双诺伐利定、吗啡-葡萄糖酸苷、去芬太尼和诺伐利定的方法。在Phenomenex C12 MAX-RP色谱柱（4 gm, 150 x 2mm）上使用甲酸铵缓冲液（pH 3.5）和乙腈梯度，分离时间为35 min。验证数据在要求的范围内。该检测方法已成功应用于真实的血浆样本，可以确认过量情况的诊断，并监测患者的依从性，特别是在姑息治疗患者中。版权所有(c) 2006约翰威利父子有限公司
A fully validated liquid chromatographic procedure coupled with electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) is presented for quantitative determination of the opioids buprenorphine, codeine, fentanyl, hydromorphone, methadone, morphine, oxycodone, oxymorphone, piritramide, tilidine, and tramadol together with their metabolites bisnortilidine, morphine-glucuronides, norfentanyl, and nortilidine in blood plasma after an automatically performed solid-phase extraction (SPE). Separation was achieved in 35 min on a Phenomenex C12 MAX-RP column (4 gm, 150 x 2 mm) using a gradient of ammonium formiate buffer (pH 3.5) and acetonitrile. The validation data were within the required limits. The assay was successfully applied to authentic plasma samples, allowing confirmation of the diagnosis of overdose situations as well as monitoring of patients' compliance, especially in patients under palliative care. Copyright (c) 2006 John Wiley & Sons, Ltd.