Development of novel dye-doped silica nanoparticles for biomarker application
Development of novel dye-doped silica nanoparticles for biomarker application
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DOI：
10.1117/1.1353590
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发表时间：
2001-04-01
影响因子：
3.5
通讯作者：
Tan, WH
中科院分区：
文献类型：
--
作者：
Santra, S;Wang, KM;Tan, WH
我们报道了一种新型发光纳米粒子的开发，该纳米粒子由无机发光染料Tris（2,2'-联吡啶基）二氯钌（II）六水化合物组成，掺杂在二氧化硅网络中。这些染料掺杂二氧化硅（DDS）纳米颗粒是利用油包水微乳液技术合成的，在该技术中，正硅酸四乙酯的受控水解导致单分散纳米颗粒的形成。制备了不同尺寸的纳米颗粒：小颗粒（5 +/-1 nm）、中颗粒（63 +/-4 nm）和大颗粒（400 +/- 10 nm），显示了微乳液技术合成均匀纳米颗粒的效率。这些纳米颗粒比细胞尺寸小得多，适用于生物标志物的应用。与大多数常用的有机染料相比，这些纳米颗粒具有高度的光稳定性。这些纳米颗粒已经通过各种显微和光谱技术进行了表征。这些纳米颗粒中的染料含量经过优化以消除自猝灭。据观察，当染料含量约为20% wt%时，达到最大发光强度。DDS纳米颗粒的二氧化硅表面可用于表面修饰和生物连接。作为一种生物标志物，DDS纳米颗粒的表面经过生化修饰，可以附着膜锚定基团，并成功应用于人类白血病细胞染色。(C) 2001光学仪器工程师学会。
We report the development of novel luminescent nanoparticles composed of inorganic luminescent dye, Tris(2,2'-bipyridyl) dichlororuthenium (II) hexahydrate, doped inside a silica network. These dye doped silica (DDS) nanoparticles have been synthesized using a water-in-oil microemulsion technique in which controlled hydrolysis of the tetraethyl orthosilicate leads to the formation of monodispersed nanoparticles. They are prepared with a variety of sizes: small (5 +/-1 nm), medium (63 +/-4 nm), and large (400 +/- 10 nm), which shows the efficiency of the microemulsion technique for the synthesis of uniform nanoparticles. All these nanoparticles are suitable for biomarker application since they are much smaller than cellular dimension. These nanoparticles are highly photostable in comparison to most commonly used organic dyes. These nanoparticles have been characterized by various microscopic and spectroscopic techniques. The amount of dye content in these nanoparticles has been optimized to eliminate self-quenching. It has been observed that maximum luminescence intensity is achieved when the dye content is around 20 wt%. Silica surface of DDS nanoparticles is available for surface modification and bioconjunction. For demonstration as a biomarker, the DDS nanoparticle's surface has been biochemically modified to attach membrane-anchoring groups and applied successfully to stain human leukemia cells. (C) 2001 Society of Photo-Optical Instrumentation Engineers.