Effects of norepinephrine on expression of IGF-1/IGF-1R and SERCA2 in rat heart.
Effects of norepinephrine on expression of IGF-1/IGF-1R and SERCA2 in rat heart.
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DOI：
10.1016/s0008-6363(97)00210-1
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发表时间：
1998
影响因子：
10.8
通讯作者：
X. Sun;Y. Ng
中科院分区：
文献类型：
--
作者：
X. Sun;Y. Ng
Objectives: The effects of norepinephrine on expression of cardiac genes during pathological cardiac growth and heart failure are not fully understood. Tissue insulin-like growth factor 1 (IGF-1) and its receptor (IGF-1R) play an important role in the regulation of the hyperplastic capacity of cardiac myocytes. Sarcoplasmic reticulum Ca2+-ATPase (SERCA2), on the other hand, is important in regulating cardiac contractile function. The present study examined the effects of elevated levels of NE on expression of IGF-1/IGF-1R and SERCA2 mRNAs.Methods: Rats were infused with NE using osmotic minipumps for 3 and 6 days at a rate of 50 μg/kg/h and also at a higher dose (130 μg/kg/h) for 6 and 14 days. Levels of expression of IGF-1/IGF-1R and SERCA2 mRNAs were determined by ribonuclease protection assay and by Northern blotting, respectively.Results: NE treatment significantly increased IGF-1 mRNA levels in both left- and right-ventricle; however, levels of IGF-1R increased in the left- but not the right-ventricle. By contrast, NE infusion at both the lower dose and the higher dose failed to alter expression of SERCA2 mRNA.Conclusion: Our results suggest that NE treatment differentially regulates expression of IGF-1 and IGF-1R in the ventricles of rat heart and that NE appears not to affect expression of SERCA2 mRNA.
