Chandelier Cartridge Density Is Reduced in the Prefrontal Cortex in Autism
Chandelier Cartridge Density Is Reduced in the Prefrontal Cortex in Autism
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DOI：
10.1093/cercor/bhaa402
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发表时间：
2021-02-01
期刊：
影响因子：
3.7
通讯作者：
Martinez-Cerdeno, Veronica
中科院分区：
文献类型：
--
作者：
Amina, Sarwat;Falcone, Carmen;Martinez-Cerdeno, Veronica
兴奋-抑制平衡的改变被认为是自闭症患者大脑皮层的共同特征，这可能是由于形成皮质回路的兴奋性和/或抑制性细胞的数量和/或功能的改变所致。我们之前发现自闭症患者前额皮质中的小清蛋白 (PV)+ 中间神经元（称为吊灯 (Ch) 细胞）数量减少。这种减少可能是由于 Ch 细胞数量减少造成的，但也可能是由于 Ch 细胞 PV 蛋白表达减少造成的。为了进一步确定自闭症患者中 Ch 细胞数量是否发生改变，我们采用与之前的研究不同的方法和不同的患者队列对 Ch 细胞数量进行了量化。我们量化了表达 GAT1（而不是 PV）的 Ch 细胞盒（而不是 Ch 细胞体体）的数量。具体来说，我们对 11 名自闭症病例和 11 名对照病例的前额叶 BA9、BA46 和 BA47 区域的 GAT1+ 墨盒进行了量化。我们发现自闭症患者所有区域和层的 GAT1+ 墨盒密度均下降。这种改变是原因还是结果尚不清楚，但可能是由于皮质产前和/或产后发育期间发生的改变造成的。
An alteration in the balance of excitation-inhibition has been proposed as a common characteristic of the cerebral cortex in autism, which may be due to an alteration in the number and/or function of the excitatory and/or inhibitory cells that form the cortical circuitry. We previously found a decreased number of the parvalbumin (PV)+ interneuron known as Chandelier (Ch) cell in the prefrontal cortex in autism. This decrease could result from a decreased number of Ch cells, but also from decreased PV protein expression by Ch cells. To further determine if Ch cell number is altered in autism, we quantified the number of Ch cells following a different approach and different patient cohort than in our previous studies. We quantified the number of Ch cell cartridges-rather than Ch cell somata-that expressed GAT1-rather than PV. Specifically, we quantified GAT1+ cartridges in prefrontal areas BA9, BA46, and BA47 of 11 cases with autism and 11 control cases. We found that the density of GAT1+ cartridges was decreased in autism in all areas and layers. Whether this alteration is cause or effect remains unclear but could result from alterations that take place during cortical prenatal and/or postnatal development.