Activation of the feeding reflex in Hydra littoralis. I. Role played by reduced glutathione and quantitative assay of the feeding reflex.
Activation of the feeding reflex in Hydra littoralis. I. Role played by reduced glutathione and quantitative assay of the feeding reflex.
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DOI：
10.1085/jgp.45.2.331
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发表时间：
1961-11
期刊：
影响因子：
--
通讯作者：
LENHOFF HM
中科院分区：
文献类型：
--
作者：
LENHOFF HM
本文介绍了一种简单而准确的定量测定水螅谷胱甘肽激活的摄食反射的方法。结果表明：（a）感受器-效应器系统的数量有限，可能位于嘴周围和触手上。(b)浓度大于5 × 10-6 M的谷胱甘肽激活所有这些系统; 10-5 M谷胱甘肽激活半最大响应。(c)谷氨酸必须持续存在于受体位点，以使反应发生。(d)由于水螅体内的一些变化，而不是由于谷胱甘肽的任何改变，反应在过量谷胱甘肽的存在下停止。本文综述了谷胱甘肽结合并激活谷胱甘肽受体引起摄食反射的机制。
A simple and accurate quantitative assay of the glutathione-activated feeding reflex in Hydra is described. The results show: (a) There are a limited number of receptor-effector systems, probably localized in the area immediately around the mouth and on the tentacles. (b) Concentrations of glutathione greater than 5 x 10-6 M activate all these systems; 10-5 M glutathione elicits a half-maximum response. (c) Glutathione must be constantly present at the receptor site in order for a response to occur. (d) The response stops in the presence of excess glutathione because of some change within the Hydra, and not as a result of any alteration to the glutathione. The present state of knowledge concerning the mechanism by which glutathione combines with and activates the glutathione receptor to elicit the feeding reflex is summarized.