Priming donor lungs with thioredoxin-1 attenuates acute allograft injury in a rat model of lung transplantation.
Priming donor lungs with thioredoxin-1 attenuates acute allograft injury in a rat model of lung transplantation.
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DOI：
10.1016/j.healun.2008.07.006
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发表时间：
2008-10
期刊：
影响因子：
--
通讯作者：
Patel JM
中科院分区：
文献类型：
--
作者：
Hu H;Lu L;Mu W;Johnson RJ;Block ER;Patel JM
Lung graft dysfunction and rejection remain a significant cause of morbidity and mortality in transplant recipients. Thioredoxin-1 (Trx), a redox-regulatory protein, has been known to function as an antioxidant against oxidative injury in multiple organs including lungs. We examined whether priming of the donor lungs with Trx prior to transplantation attenuates acute lung injury. Orthotopic left lung transplantation was performed from Lewis (donor) to Sprague-Dawley (recipient) rats using the cuff technique. For Trx priming, the donor lungs were perfused and stored in Perfadex solution with or without the presence of purified Trx prior to transplantation. Changes in bronchoalveolar (BAL) fluid analysis, allograft oxygen exchange function, nuclear factor kappa B (NF-kB)/DNA binding, myeloperoxidase (MPO) activities, and immunohisotologic evaluation of neutrophils, macrophages and cytotoxic T-cells (CD8+) infiltration were examined in one and/or five day post-transplant allograft (left) and native (right) lungs. BAL cell differential analysis showed significant increases in macrophages and neutrophils in one day post-transplant whereas lymphocyte infiltration was significantly increased in both one and five days post transplant allografts. MPO and NF-kB/DNA binding activities were increased over basal activities one and five days post transplant. Immunohistology staining of one and five day post transplant allografts revealed increased infiltration of macrophages, neutrophils, and CD8+ T cell subsets. Priming of donor lungs with Trx prior to transplantation improved O2 exchange and attenuated NF-kB/DNA binding activity and infiltration of macrophages, neutrophils, and CD8+ T cell subsets in one and five day post transplant allografts. Priming of donor lungs with Trx prior to transplantation attenuates acute allograft injury in a rat model of lung transplantation. This protection appears to be associated with Trx’s antioxidant function that limits early I/R injury, NF-kB activation, and progressive infiltration of inflammatory and immune cells in allografts.
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DOI：
10.1084/jem.185.11.1897
发表时间：
1997-06-02
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Boothby MR;Mora AL;Scherer DC;Brockman JA;Ballard DW
通讯作者：
Ballard DW
影响因子：
38.9
作者：
Cottini, SR;Lerch, N;Ricou, B
通讯作者：
Ricou, B
影响因子：
13.6
作者：
Mu, W;Ouyang, X;Johnson, RJ
通讯作者：
Johnson, RJ
DOI：
10.1016/s0022-5223(97)70393-3
发表时间：
1997-01-01
影响因子：
6
作者：
Okubo, K;Kosaka, S;Wada, H
通讯作者：
Wada, H
影响因子：
9.6
作者：
Christie, JD;Bavaria, JE;Kotloff, RM
通讯作者：
Kotloff, RM