Assessment of purity of rice hybrids using microsatellite and STS markers
Assessment of purity of rice hybrids using microsatellite and STS markers
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DOI：
10.2135/cropsci2002.1369
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发表时间：
2002-07
期刊：
影响因子：
2.3
通讯作者：
J. Yashitola;T. Thirumurugan;R. Sundaram;M. K. Naseerullah;M. Ramesha;N. P. Sarma;R. V. Sonti
中科院分区：
文献类型：
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作者：
J. Yashitola;T. Thirumurugan;R. Sundaram;M. K. Naseerullah;M. Ramesha;N. P. Sarma;R. V. Sonti
杂交水稻种子纯度的鉴定通常通过生长试验（growouttest，GOT）进行，该试验基于对生长至成熟的植物的形态和花特征的评估。对于种子公司来说，在等待GOT结果的同时，大量的资本被锁定在杂交种子库存的形式上。为了用基于DNA的检测取代GOT，已经通过微卫星和序列标记位点（STS）多态性来筛选细胞质雄性不育（CMS）、恢复系和杂交系。一个简单的程序检测杂合性和纯度已被标准化，并使用6日龄水稻（Oryza sativa L.）幼苗，可用于杂种种子批中的异型检测。对杂交水稻亲本的杂合程度进行了评估，结果表明，一个单一的，适当选择的微卫星标记应该足以评估杂交种子纯度。
The estimation of hybrid rice seed purity is done conventionally by the grow out test (GOT), which is based on the assessment of morphological and floral characteristics in plants grown to maturity. For seed companies, large amounts of capital are locked up in the form of hybrid seed stock while awaiting the results of the GOT. With the objective of replacing the GOT with DNA based assays, cytoplasmic male sterile (CMS), restorer, and hybrid lines have been screened by means of microsatellite and sequence tagged site (STS) polymorphisms. A simple procedure for detecting heterozygosity and purity has been standardized and uses 6-d-old rice (Oryza saliva L.) seedlings, which could be used for detection of off-types in hybrid seed lots. The extent of heterozygosity within parental lines of rice hybrids was assessed and the results suggest that a single, appropriately chosen microsatellite marker should be sufficient for assessing hybrid seed purity.