The expression of diacylglycerol kinase theta during the organogenesis of mouse embryos.
The expression of diacylglycerol kinase theta during the organogenesis of mouse embryos.
复制标题
小鼠胚胎的器官发生过程中二酰基甘油激酶的表达。
DOI：
10.1186/1471-213x-13-35
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发表时间：
2013-10-01
影响因子：
--
通讯作者：
Shirai Y
中科院分区：
文献类型：
--
作者：
Ueda S;Tu-Sekine B;Yamanoue M;Raben DM;Shirai Y
甘油二酯激酶（DGK）是调节甘油二酯（DG）周转的关键酶，参与多种生理功能。DGKθ具有独特的结构域，是V型DGK的唯一成员。为了揭示DGKθ的时空表达，我们对小鼠胚胎的石蜡切片进行了免疫组织化学染色。在发育早期（E10.5和E11.5），DGKθ在脑、脊髓、背根神经节和肢芽中有显著表达，在心球、肝原基和肠原基中也有中等表达。在晚期（E12.5和14.5），新皮质、上丘脑、下丘脑、延髓和脑桥中的DGKθ表达持续或增加。DGKθ在表皮、口咽膜、消化道和支气管的几乎所有上皮中也很明显。在胚胎发育前期（E16.5和E18.5），DGKθ在中枢神经系统、肠和肾中的表达模式得以维持，但在分化的表皮中表达减弱。这些结果表明，DGKθ不仅在脑中，而且在胚胎期的多种器官和组织中可能发挥重要的生理作用。
Diacylglycerol kinase (DGK) is a key enzyme that regulates diacylglycerol (DG) turnover and is involved in a variety of physiological functions. The isoform DGKθ has a unique domain structure and is the sole member of type V DGK. To reveal the spatial and temporal expression of DGKθ we performed immunohistochemical staining on paraffin sections of mouse embryos. At an early stage of development (E10.5 and 11.5), the expression of DGKθ was prominently detected in the brain, spinal cord, dorsal root ganglion, and limb bud, and was also moderately detected in the bulbus cordis and the primordium of the liver and gut. At later stages (E12.5 and 14.5), DGKθ expression persisted or increased in the neocortex, epithalamus, hypothalamus, medulla oblongata, and pons. DGKθ was also evident in the epidermis, and nearly all epithelia of the oropharyngeal membrane, digestive tract, and bronchea. At prenatal developmental stages (E16.5 and E18.5), the expression pattern of DGKθ was maintained in the central nervous system, intestine, and kidney, but was attenuated in the differentiated epidermis. These results suggest that DGKθ may play important physiological roles not only in the brain, but also in diverse organs and tissues during the embryonic stages.