Structural organization and functional properties of miniature DNA insertion sequences in yersiniae
Structural organization and functional properties of miniature DNA insertion sequences in yersiniae
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DOI：
10.1128/jb.00942-06
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发表时间：
2006-11-01
影响因子：
3.2
通讯作者：
Di Nocera, Pier Paolo
中科院分区：
文献类型：
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作者：
De Gregorio, Eliana;Silvestro, Giustina;Di Nocera, Pier Paolo
YPAL（耶尔森氏菌回文序列）是分散在耶尔森氏菌染色体上的微型DNA插入。这些基因间重复序列的传播可能通过转座发生，如在其末端存在靶位点重复和鉴定在耶尔森氏菌菌株中存在/不存在YPAL DNA的同线染色体区域所示。YPAL倾向于紧密插入侧翼基因的终止密码子下游，并且许多YPAL靶标与ρ非依赖性转录终止子样序列重叠。这种特殊的插入模式支持了这样的假设，即大多数重复序列与上游序列共转录成mRNA。YPAL RNA折叠成稳定的发夹，其可调节mRNA衰变。因此，我们发现YPAL阳性转录物在小肠结肠炎耶尔森氏菌细胞中的积累水平显著高于在其3'非翻译区缺乏YPAL序列的同源转录物。
YPALs (Yersinia palindromic sequences) are miniature DNA insertions scattered along the chromosomes of yersiniae. The spread of these intergenic repeats likely occurred via transposition, as suggested by the presence of target site duplications at their termini and the identification of syntenic chromosomal regions which differ in the presence/absence of YPAL DNA among Yersinia strains. YPALs tend to be inserted closely downstream from the stop codon of flanking genes, and many YPAL targets overlap rho-independent transcriptional terminator-like sequences. This peculiar pattern of insertion supports the hypothesis that most of these repeats are cotranscribed with upstream sequences into mRNAs. YPAL RNAs fold into stable hairpins which may modulate mRNA decay. Accordingly, we found that YPAL-positive transcripts accumulate in Yersinia enterocolitica cells at significantly higher levels than homologous transcripts lacking YPAL sequences in their 3' untranslated region.