Genetic and molecular analysis of utility of sd1 alleles in rice breeding
Genetic and molecular analysis of utility of sd1 alleles in rice breeding
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DOI：
10.1270/jsbbs.57.53
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发表时间：
2007-03-01
期刊：
影响因子：
2.4
通讯作者：
Ashikari, Motoyuki
中科院分区：
文献类型：
--
作者：
Asano, Kenji;Takashi, Tomonori;Ashikari, Motoyuki
20世纪60年代，高产半矮秆水稻品种IR8的广泛采用，在亚洲引发了一场“绿色革命”。该品种的矮小是由于编码GA20氧化酶-2(GA20ox-2)的SD1基因功能丧失所致，GA20ox-2催化赤霉素生物合成的后期步骤。在这项研究中，我们研究了SD1突变在水稻半矮秆品种产生中的广泛应用。遗传和分子分析表明，IRS的SD1等位基因已被用于生产中。对57个半矮秆水稻品种的sd1基因座序列分析表明，至少有7个sd1等位基因在中国、美国和日本的半矮秆水稻品种选育中被利用。利用如此多的不同等位基因同时控制相同的目标性状，突显了GA20ox-2的突变诱导了水稻在农学上的有利结构。
The widespread adoption of the high-yielding semi-dwarf rice variety, IR8, led to the "green revolution" in Asia in the 1960s. The short stature of this variety is due to a loss-of-function of the SD1 gene that encodes a GA20 oxidase-2 (GA20ox-2) which catalyzes late steps of gibberellin biosynthesis. In this study, we investigated how widely sd1 mutations have been employed in the generation of semi-dwarf varieties of rice. Genetic and molecular analyses revealed that the sd1 allele of IRS has been used in the production of japonica varieties. Sequence analysis of the SD1 locus of 57 semi-dwarf varieties showed that at least 7 sd1 alleles have been used in the breeding of semi-dwarf rice varieties in China, USA and Japan. The utilization of such a high number of different alleles all controlling the same target trait highlights that mutations in GA20ox-2 induce an agronomically advantageous architecture in rice.