Differential gene expression profiling in the developed ovaries between the parthenogenetic and bisexual female rice water weevils, Lissorhoptrus oryzophilus Kuschel (Coleoptera: Curculionidae)
Differential gene expression profiling in the developed ovaries between the parthenogenetic and bisexual female rice water weevils, Lissorhoptrus oryzophilus Kuschel (Coleoptera: Curculionidae)
复制标题
DOI：
10.1007/s11434-009-0537-0
复制
发表时间：
2009-10-01
影响因子：
--
通讯作者：
Way, M. O.
中科院分区：
文献类型：
--
作者：
Yang Pu;Zhou WenWu;Way, M. O.
稻水象甲Lissorhoptrus oryzophilus Kuschel（鞘翅目：粟象科）在美国东南部通过性别繁殖，但在加利福尼亚和其他亚洲和欧洲的入侵地区通过孤雌生殖繁殖。本研究的目的是建立稻水象甲孤雌生殖基因表达谱，以便更好地了解稻水象甲孤雌生殖的分子机制。采用抑制减法杂交（SSH）技术分析了单性生殖稻水象甲和两性稻水象甲雌性稻水象甲发育后卵巢中基因表达的差异。总共获得了70个contigs， BLASTX搜索确定了28个具有差异功能的基因。根据孤雌生殖的细胞学过程，选择微管蛋白α -1链和信号转导基因等进行实时定量RT-PCR分析，并探讨其可能与孤雌生殖的分子机制相关的功能。微管蛋白α -1链和一些信号转导基因可能与水稻水象甲孤雌生殖的分子机制有关。
The rice water weevil, Lissorhoptrus oryzophilus Kuschel (Coleoptera: Curculionidae), reproduces by sex in the Southeastern United States, but reproduces by parthenogenesis in California and other invaded regions in Asia and Europe. The objective of this study was to create a parthenogenetic gene expression profile of the rice water weevil in order to gain a better insight into the molecular mechanisms of parthenogenesis in the weevil. Suppression subtractive hybridization (SSH) technique was employed for profiling differential gene expression in the developed ovary between the parthenogenetic and bisexual female rice water weevils. A total of 70 contigs were obtained, and the BLASTX search identified putatively 28 genes with differential functions. According to the cytological process of parthenogenesis, the tubulin alpha-1 chain and signal transduction genes etc. were selected for real time quantitative RT-PCR analyses, and their possible functions related to the molecular mechanism of parthenogenesis were discussed. The tubulin alpha-1 chain and some signal transduction genes may be related to the molecular mechanisms of parthenogenesis of the rice water weevil.