THE NONOSTEOGENIC MOUSE PLURIPOTENT CELL-LINE, C3H10T1/2, IS INDUCED TO DIFFERENTIATE INTO OSTEOBLASTIC CELLS BY RECOMBINANT HUMAN BONE MORPHOGENETIC PROTEIN-2
THE NONOSTEOGENIC MOUSE PLURIPOTENT CELL-LINE, C3H10T1/2, IS INDUCED TO DIFFERENTIATE INTO OSTEOBLASTIC CELLS BY RECOMBINANT HUMAN BONE MORPHOGENETIC PROTEIN-2
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DOI：
10.1016/s0006-291x(05)80208-6
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发表时间：
1990-10-15
影响因子：
3.1
通讯作者：
SUDA, T
中科院分区：
文献类型：
--
作者：
KATAGIRI, T;YAMAGUCHI, A;SUDA, T
在体外研究了多种激素和细胞因子诱导非成骨小鼠多能细胞系C3H10T1/2 （10T1/2）向成骨细胞分化的可能性。在测试的许多药物中，重组人骨形态发生蛋白-2 （rhBMP-2）和维甲酸诱导10T1/2细胞的碱性磷酸酶（ALP）活性。rhBMP-2还能诱导细胞中ALP mRNA的表达。地塞米松,1. alpha。25-二羟基维生素D3，转化生长因子- β。胰岛素样生长因子- 1和胰岛素样生长因子- 1不刺激ALP活性。在10T1/2细胞中，用rhBMP-2处理可显著诱导甲状旁腺激素产生cAMP。rhBMP-2和维甲酸均未诱导NIH3T3成纤维细胞的ALP活性。这些结果表明，在BMP-2的调控下，10T1/2细胞具有向成骨细胞分化的潜力。
The possibility that the non-osteogenic mouse pluripotent cell line, C3H10T1/2 (10T1/2), could be induced to differentiate into osteogenic cells by various hormones and cytokines was examined in vitro. Of a number of agents tested, recombinant human bone morphogenetic protein-2 (rhBMP-2) and retinoic acid induced alkaline phosphatase (ALP) activity in 10T1/2 cells. rhBMP-2 also induced mRNA expression of ALP in the cells. Dexamethasone, 1.alpha.,25-dihydroxyvitamin D3, transforming growth factor-.beta.1 and insulin-like growth factor-I did not stimulate ALP activity. Treatment with rhBMP-2 greatly induced cAMP production in response to parathyroid hormone in 10T1/2 cells. No ALP activity was induced in NIH3T3 fibroblasts treated with rhBMP-2 or retinoic acid. These results indicate that 10T1/2 cells have a potential to differentiate into osteogenic cells under the control of BMP-2.