Cell proliferation and specialization during endochondral osteogenesis in young rats.
Cell proliferation and specialization during endochondral osteogenesis in young rats.
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DOI：
10.1083/jcb.14.3.357
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发表时间：
1962-09
期刊：
影响因子：
--
通讯作者：
YOUNG RW
中科院分区：
文献类型：
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作者：
YOUNG RW
在32只Long-Evans大鼠的肋骨和胫骨中进行放射自显影研究，这些大鼠在6日龄时注射1 µc/gm H3-胸苷，并在1小时至2周后间隔处死。通过分析（a）标记的有丝分裂的百分比，（B）骨细胞中标记的细胞核的百分比，和（c）颗粒计数来研究骨细胞的增殖和特化。得出以下结论：不同类型的骨细胞代表同一细胞的不同功能状态。细胞分裂通常局限于形态上非特化的“骨祖细胞”状态。特化骨细胞通过骨祖细胞的调节而产生。干骺端细胞平均传代时间最短，骨膜细胞平均传代时间最长，骨内膜细胞平均传代时间居中。DNA合成的平均持续时间相对恒定（约8小时）。随着世代时间的增加，G2 +有丝分裂略有增加，但主要变化是G1期延长。分裂后，骨祖细胞状态的细胞可以保留在祖细胞库中或经历细胞类型的调节，特化为成骨细胞或并入破骨细胞。
Endochondral osteogenesis was studied autoradiographically in ribs and tibiae of 32 Long-Evans rats injected with 1 µc/gm H3-thymidine at 6 days of age and sacrificed at intervals between 1 hour and 2 weeks later. Proliferation and specialization of bone cells were studied by analyses of (a) the percentage of mitoses which were labeled, (b) the percentage of labeled nuclei in bone cells, and (c) grain counts. The following conclusions were derived: The various types of bone cells represent different functional states of the same cell. Cell division is usually restricted to cells in the morphologically unspecialized "osteoprogenitor" state. Specialized bone cells arise by modulation of osteoprogenitor cells. The average cell generation time is shortest in the metaphysis, longest in the periosteum, and intermediate in the endosteum. The average duration of DNA synthesis is relatively constant (about 8 hours). With increasing length of generation time there is a slight increase in G2 + mitosis, but the major change is a lengthening of G1. After dividing, cells in the osteoprogenitor state may remain within the progenitor pool or undergo modulation of cell type, specializing as osteoblasts or becoming incorporated in osteoclasts.