Secretory IgA antibodies provide cross-protection against infection with different strains of influenza B virus
Secretory IgA antibodies provide cross-protection against infection with different strains of influenza B virus
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DOI：
10.1002/jmv.20173
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发表时间：
2004-10-01
影响因子：
12.7
通讯作者：
Sata, T
中科院分区：
文献类型：
--
作者：
Asahi-Ozaki, Y;Yoshikawa, T;Sata, T
本研究检测了分泌型伊加（S-IgA）抗体（Abs）是否可以赋予针对抗原性不同谱系的B型流感病毒感染的交叉保护性免疫。通过用不同的B病毒感染或通过鼻内（i. n.）接种不同的灭活疫苗。四周后，用代表B/维多利亚/2/87（B/维多利亚）-谱系的B/茨木/2/85病毒或代表B/Yamagata-谱系的B/Yamagata/ 16/88病毒攻击小鼠.攻毒后3天，测定鼻洗液和血清标本中攻毒病毒抗原特异性伊加和IgG抗体以及抗攻毒病毒的保护作用。在野生型小鼠，B/茨木（或B/山形）交叉反应伊加抗体检测在较高的水平时，感染或免疫同源谱系病毒和较低的水平时，感染或免疫异源谱系病毒。鼻腔交叉反应性伊加Ab的量与同源谱系病毒的交叉保护效力之间存在相关性。在缺乏pIgR的小鼠中，在接种疫苗的小鼠中仅少量检测到鼻交叉保护性伊加Ab，并观察到伊加在血清中的蓄积。这种鼻伊加的减少伴随着对B/茨木（或B/Yamagata）病毒感染的无效交叉保护。这些结果表明，攻击病毒抗原交叉反应的S-IgA鼻分泌物诱导的i. n。感染或疫苗接种涉及提供交叉保护以对抗B/维多利亚-或B/Yamagata-谱系内的病毒攻击感染。
This study examined whether secretory IgA (S-IgA) antibodies (Abs) could confer cross-protective immunity against infection with influenza B viruses of antigenically distinct lineages. Wildtype or polymeric Ig receptor (pIgR)-knockout (KO) mice were immunized by infection with different B viruses or by intranasal (i.n.) administration with different inactivated vaccines. Four weeks later mice were challenged with either the B/Ibaraki/2/85 virus, representative of the B/ Victoria/2/87 (B/Victoria)-lineage, or B/Yamagata/ 16/88 virus, representative of the B/Yamagata- lineage. Three days after challenge, nasal wash and serum specimens were assayed for IgA and IgG Abs specific for challenge viral antigens and for protection against challenge viruses. In wildtype mice, B/Ibaraki (or B/Yamagata) cross-reactive IgA Abs were detected at higher levels when infected or immunized with homologous-lineage viruses and at lower levels when infected or immunized with heterologous-lineage viruses. There was a correlation between the amount of nasal cross-reactive IgA Ab and the efficacy of cross-protection with a homologous-lineage virus. In mice lacking the pIgR, nasal cross-protective IgA Abs were only marginally detected in vaccinated mice and an accumulation of IgA in the serum was observed. This reduction of nasal IgA was accompanied by inefficient cross-protection against the B/Ibaraki (or B/Yamagata) virus infection. These results suggest that challenge viral-antigen cross-reactive S-IgA in nasal secretions induced by i.n. infection or vaccination is involved in providing cross-protection against challenge infection with virus within either the B/ Victoria- or B/Yamagata-lineage.