Isolation and characterization of a new mouse mammary tumor virus from BALB/c mice.
Isolation and characterization of a new mouse mammary tumor virus from BALB/c mice.
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从 BALB/c 小鼠中分离和表征新型小鼠乳腺肿瘤病毒。
DOI：
10.1016/0042-6822(81)90263-4
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发表时间：
1981
期刊：
影响因子：
3.7
通讯作者：
Schlom,J
中科院分区：
文献类型：
--
作者：
Drohan,W;Teramoto,YA;Medina,D;Schlom,J
从BALB/cCrl Med小鼠亚系BALB/cV中分离到一种新的小鼠乳腺肿瘤病毒（MMTV）。尽管亲本BALB/cCrl Med群体的繁殖雌性动物的乳腺肿瘤发生率为1%，但BALB/cV亚系的繁殖雌性动物中有47%在10月龄前发生乳腺肿瘤。寄养实验表明，这种病毒，称为MMTV（BALB/c），只通过牛奶传播。通过对MMTV 28，000-道尔顿主要核心蛋白（p28）和52，000（gp 52）-和36，000-道尔顿（gp 36）主要包膜糖蛋白的一系列竞争性放射免疫测定，显示新型MMTV分离株与C3 H、GR和RIII小鼠的MMTV变体在免疫学上相关，但不同。针对MMTV gp 36的单克隆抗体也用于清楚地区分MMTV（BALB/c）与MMTV（C3 H）、MMTV（RIII）、MMTV（GR）、MMTV（C3 HfC 57 BL）和MMTV（A）。用限制性内切酶和Southern印迹技术检测了BALB/cV亚系中乳腺癌的MMTV特异性前病毒DNA含量，并与BALB/cAnde乳腺肿瘤的MMTV前病毒DNA含量进行了比较。从这些肿瘤中产生的病毒被称为MMTV（O）。两种乳腺肿瘤的高分子量DNA的EcoRI酶切分析表明，与正常BALB/c组织的DNA相比，存在额外的MMT相关前病毒序列。用限制性内切核酸酶SacI产生的模式将BALB/cV小鼠乳腺肿瘤中的额外MMTV特异性前病毒信息与含有GR、C3 H或RIII MMTV的组织中的前病毒信息以及BALB/cAnDe乳腺肿瘤中的前病毒信息区分开来。因此，这些免疫学和分子研究将MMTV（BALB/c）定义为新的MMTV变体。
A novel mouse mammary tumor virus (MMTV) has been isolated from mice of a subline of the BALB/cCrl Med mouse strain designated BALB/cV. Whereas breeding females of the parent BALB/cCrl Med colony have a mammary tumor incidence of 1%, 47% of the breeding females of the BALB/cV subline develop mammary tumors before 10 months of age. Foster nursing experiments demonstrated this virus, termed MMTV (BALB/c), was transmitted only by milk. The novel MMTV isolate was shown to be immunologically related to, but distinct, from the MMTV variants of C3H, GR, and RIII mice by a series of competition radioimmunoassays for the MMTV 28,000-dalton major core protein (p28), and the 52,000 (gp52)- and 36,000-dalton (gp36) major envelope glycoproteins. Monoclonal antibodies directed against MMTV gp36 were also used to clearly distinguish MMTV(BALB/c) from MMTV(C3H), MMTV(RIII), MMTV(GR), MMTV(C3HfC57BL), and MMTV(A). MMTV-specific proviral DNA content of mammary adenocarcinomas arising in the BALB/cV subline was examined with restriction endonucleases and the Southern blot technique, and compared to the MMTV proviral DNA content of BALB/cAnDe mammary tumors. The virus arising from these latter tumors has been termed MMTV(O). Analysis ofEcoRI digests of high-molecular-weight DNA from both types of mammary tumors demonstrated additional MMTV-related proviral sequences when compared to the DNA of normal BALB/c tissues. The patterns generated with the restriction endonucleaseSacI distinguished the additional MMTV-specific proviral information in the mammary tumors of the BALB/cV mice from the proviral information in tissues containing the GR, C3H, or RIII MMTVs, as well as from the proviral information in the BALB/cAnDe mammary tumors. These immunological and molecular studies thus define MMTV(BALB/c) as a novel MMTV variant.