The PEG-responding desiccome of the alder microsymbiont Frankia alni.
The PEG-responding desiccome of the alder microsymbiont Frankia alni.
复制标题
DOI：
10.1038/s41598-017-18839-0
复制
发表时间：
2018-01-15
影响因子：
4.6
通讯作者：
Sghaier H
中科院分区：
文献类型：
--
作者：
Ghedira K;Harigua-Souiai E;Ben Hamda C;Fournier P;Pujic P;Guesmi S;Guizani I;Miotello G;Armengaud J;Normand P;Sghaier H
放线菌植物具有重要的生态和经济价值。与固氮细菌的共生使这些木本双子叶植物能够在氮缺乏、水分胁迫或其他极端条件下在土壤中定居。然而，参与共生微生物耐旱性的蛋白质尚未被鉴定。在这里，我们从地理上分布最广的革兰氏阳性固氮植物共生体，Frankia alni，通过下一代蛋白质组学，利用Q-Exactive HF串联质谱仪配备超高场Orbitrap分析仪的聚乙二醇（PEG）响应干燥。共检测和定量了2，052种蛋白质。在渗透胁迫下，PEG培养的F. alni细胞增加了与蛋白质相关的蛋白质的丰度，如ABC转运蛋白、机械敏感离子通道和重复的规则间隔短回文重复序列CRISPR相关（cas）组分。同时，固氮、好氧呼吸和同源重组等代谢途径也显著下调。分子模拟和对接模拟表明，PEG是作用于Frankia的部分填充内部的一个上调的抗压应力大电导mechanosensitive通道。
Actinorhizal plants are ecologically and economically important. Symbiosis with nitrogen-fixing bacteria allows these woody dicotyledonous plants to colonise soils under nitrogen deficiency, water-stress or other extreme conditions. However, proteins involved in xerotolerance of symbiotic microorganisms have yet to be identified. Here we characterise the polyethylene glycol (PEG)-responding desiccome from the most geographically widespread Gram-positive nitrogen-fixing plant symbiont, Frankia alni, by next-generation proteomics, taking advantage of a Q-Exactive HF tandem mass spectrometer equipped with an ultra-high-field Orbitrap analyser. A total of 2,052 proteins were detected and quantified. Under osmotic stress, PEG-grown F. alni cells increased the abundance of envelope-associated proteins like ABC transporters, mechano-sensitive ion channels and Clustered Regularly Interspaced Short Palindromic Repeats CRISPR-associated (cas) components. Conjointly, dispensable pathways, like nitrogen fixation, aerobic respiration and homologous recombination, were markedly down-regulated. Molecular modelling and docking simulations suggested that the PEG is acting on Frankia partly by filling the inner part of an up-regulated osmotic-stress large conductance mechanosensitive channel.
登录
查看更多内容
影响因子：
7
作者：
Clair, Geremy;Armengaud, Jean;Duport, Catherine
通讯作者：
Duport, Catherine
影响因子：
3.3
作者：
Cherif, Hanene;Marasco, Ramona;Ouzari, Hadda
通讯作者：
Ouzari, Hadda
影响因子：
3
作者：
Harigua-Souiai, Emna;Cortes-Ciriano, Isidro;Bouvier, Guillaume
通讯作者：
Bouvier, Guillaume
影响因子：
11
作者：
Carro, Lorena;Pujic, Petar;Normand, Philippe
通讯作者：
Normand, Philippe
DOI：
10.1007/978-1-4939-1261-2_16
发表时间：
2014-01-01
期刊：
HOST-BACTERIA INTERACTIONS: METHODS AND PROTOCOLS
影响因子：
--
作者：
Hartmann, Erica Marie;Allain, Francois;Armengaud, Jean
通讯作者：
Armengaud, Jean