CD8+ T-CELLS IN PSORIATIC LESIONS PREFERENTIALLY USE T-CELL RECEPTOR V(BETA)3 AND/OR V(BETA)13.1 GENES
CD8+ T-CELLS IN PSORIATIC LESIONS PREFERENTIALLY USE T-CELL RECEPTOR V(BETA)3 AND/OR V(BETA)13.1 GENES
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DOI：
10.1073/pnas.91.20.9282
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发表时间：
1994-09-27
影响因子：
11.1
通讯作者：
BROSTOFF, SW
中科院分区：
文献类型：
--
作者：
CHANG, JCC;SMITH, LR;BROSTOFF, SW
银屑病是一种炎性皮肤病，其特征是表皮角质形成细胞过度增殖与细胞浸润。有证据表明活化的T细胞在银屑病斑块形成中起作用。我们研究了银屑病皮损刮皮活检中表皮T细胞的T细胞受体β链可变基因片段（V-β）的使用。我们的研究结果显示，在大多数研究患者的病变中，CD 8（+）T细胞中V（β）3和/或V（β）13.1信息的表达增加，但CD 4（+）T细胞中没有。对来自皮肤的这两个V（β）基因的互补决定区3（CDR 3）的序列分析表明具有单克隆性或明显的寡克隆性。3.5-8个月后，在4名患者中进行的相同或不同病变的第二次活检再次显示V（beta）3和/或V（beta）13.1表达和克隆性增加。此外，在四名患者中的三名中，在两个活检中发现了相同的V（β）CDR 3重排，尽管患者之间没有V（β）CDR 3同源性。在其中V（beta）3和/或V（beta）13.1没有增加的两个患者中，发现V（beta）17基因使用和克隆性增加。在没有消退的病变中，携带V（β）3和/或V（β）13.1的CD 8（+）T细胞的持续存在表明它们作为效应细胞而不是调节细胞的作用。
Psoriasis is an inflammatory skin disorder characterized by epidermal keratinocyte hyperproliferation in association with a cellular infiltrate. There is evidence that activated T cells play a role in psoriatic plaque formation. We examined the T-cell receptor beta-chain variable gene segment (V-beta) use of epidermal T cells in shave biopsies of psoriatic lesions. Our results show increased expression of V (beta)3 and/or V (beta)13.1 messages in the CD8(+), but not CD4(+), T cells in the lesions of a majority of patients studied. Sequence analysis of complementarity-determining region 3 (CDR3) of these two V (beta) genes from the skin demonstrated monoclonality or marked oligoclonality. A second biopsy from the same or different lesions, performed 3.5-8 months later in four patients, again revealed increased V (beta)3 and/or V (beta)13.1 expression and clonality. Moreover, in three of the four patients, the same V (beta) CDR3 rearrangement was found in both biopsies, although there was no V (beta) CDR3 homology between patients. In two patients in which V (beta)3 and/or V (beta)13.1 was not increased, an increase in V (beta)17 gene use and clonality was found. The clonality of V (beta) sequence data indicates these cells are recruited and expanded in situ. The persistence of V (beta)3-and/or V (beta)13.1-bearing CD8(+) T cells in lesions that did not undergo resolution suggests their role as effector cells rather than as regulatory cells.