Influence of Sanao Tang on urine volume and expression of aquaporin 2 in rats with respiratory function impairment induced by passive smoking and lipopolysaccharide.
Influence of Sanao Tang on urine volume and expression of aquaporin 2 in rats with respiratory function impairment induced by passive smoking and lipopolysaccharide.
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DOI：
10.1016/s0254-6272(17)30010-9
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发表时间：
2016-12
影响因子：
2.6
通讯作者：
Zhang Jinchao;S. Yan;Z. Shujing;Gao Yushan;Wang Zhenyi;L. Xiaolei;Yan Jiamin;Li Yuhang
中科院分区：
文献类型：
--
作者：
Zhang Jinchao;S. Yan;Z. Shujing;Gao Yushan;Wang Zhenyi;L. Xiaolei;Yan Jiamin;Li Yuhang
目的观察三拗汤对被动吸烟和脂多糖所致肺功能不全大鼠尿量及肺组织水通道蛋白2（Aquaporin-2，AQP 2）表达的影响。吸烟和脂多糖（LPS）暴露诱导的大鼠呼吸功能障碍模型。SAT组采用中药水煎液灌胃。采用Anires 2005系统进行肺功能分析。采用代谢笼法收集大鼠尿液。采用酶联免疫吸附法（ELISA）测定血清、肺、肾组织中抗利尿激素（ADH）、血管紧张素II（Ang II）、心钠素（ANP）、内皮素1（ET-1）、一氧化氮（NO）、前列腺素E2（PGE 2）的含量。结果（a）与正常对照组比较，实验组大鼠用力肺活量（FVC）、第1秒用力呼气量/用力肺活量（%）、第2秒用力呼气量/用力肺活量（%）、第3秒用力呼气量/用力肺活量（%）、第4秒用力呼气量/用力肺活量（%）、第5秒用力呼气量/用力肺活量（%）、第6秒用力呼气量/用力肺活量（%）、第7秒用力呼气量/用力肺活量（%）、第10秒用力呼气量/用力肺活量（%）、降低FEV 1/FVC%、24 h尿NO和PGE 2含量，降低AQP 2 mRNA水平和ADH、Agn II、模型组血浆ANP、ET-1水平升高，差异有统计学意义（P< 0.05）。(b)与模型组比较，SAT组FVC、FEV 1、FEV 1/FVC%、24 h尿量、PGE 2和NO含量降低，AQP 2 mRNA水平、ANP、ADH和Ang II含量降低，差异有统计学意义结论舒喘灵能有效调节模型大鼠尿量; ADH、Ang Ⅱ、ANP、ET-1、NO和PGE 2可能在肺对尿量的调节中起重要作用。这可能是中医肺主水道的作用机制。
OBJECTIVETo investigate the effect of Sanao Tang (SAT) on urine volume and the expression of aquaporin-2 (AQP2) in rats with lung dysfunction induced by passive smoking and lipopolysaccharide.METHODSTotally 45 healthy Specific pathogen Free Wistar Rats were randomized into 3 groups: normal control group, model group and SAT group. A rat model of respiratory dysfunction induced by exposure to cigarette smoking and lipopolysaccharide (LPS). Lavage of decoction of the Chinese medicine was performed for rats in the SAT group. Anires 2005 System was used to analyze the pulmonary function. Urine of rats was collected through metabolism cage method. Enzyme-linked immunosorbent assay (ELISA) was used to determine content of antidiuretic hormone (ADH), angiotensin II (Ang II), atrial natriuretic factor (ANP), endothelin 1 (ET-1), nitric oxide (NO) and prostaglandin E2 (PGE2) in serum, lung and kidney. The expression of AQP2 in rat renal tissue was determined with real-time fluorescence quantitative PCR (RT-PCR).RESULTS(a) In comparison with the normal control, It was found that enforced vital capacity (FVC), 1-second forced expiratory volume/forced vital capacity% (FEV1/FVC%), 24 h urine volume content of NO and PGE2 were decreased, while AQP2mRNA level and content of ADH, Agn II, ANP and ET-1 were increased in the model group with statistical significance (P< 0.05). (b) In comparison with the model group, It was found that FVC, FEV1, FEV1/FVC%, 24 h urine volume, content of PGE2 and NO decreased, while AQP2mRNA level, content of ANP, ADH and Ang II decreased in the SAT group with statistical significance (P< 0.05).CONCLUSIONSAT might effectively regulate the urine volume in the modeled rats; ADH, Ang II, ANP, ET-1, NO and PGE2 might play an important role in the regulation on urine volume by lungs. This might be the mechanisms underpinning the function of lung governing water passage in terms of the theory of Traditional Chinese Medicine.