Strength training increases insulin-mediated glucose uptake, GLUT4 content, and insulin signaling in skeletal muscle in patients with type 2 diabetes
Strength training increases insulin-mediated glucose uptake, GLUT4 content, and insulin signaling in skeletal muscle in patients with type 2 diabetes
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DOI：
10.2337/diabetes.53.2.294
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发表时间：
2004-02-01
期刊：
影响因子：
7.7
通讯作者：
Dela, F
中科院分区：
文献类型：
--
作者：
Holten, MK;Zacho, M;Dela, F
对于 2 型糖尿病患者来说，力量训练是耐力训练的替代方案。人们对胰岛素作用和骨骼肌关键蛋白质的影响知之甚少，并且所需的力量训练量也是未知的。总共 10 名 2 型糖尿病受试者和 7 名健康男性（对照受试者）每周对一条腿进行 3 次力量训练，持续 6 周，而另一条腿则不进行训练。每节课持续时间不超过30分钟。力量训练后进行肌肉活检，并进行等血糖高胰岛素钳夹结合双腿动股静脉插管。一般来说，两组都获得了性质相似的反应。在夹紧过程中，训练过的腿与未训练过的腿相比，腿部血流量较高（P < 0.05），但尽管如此，训练过的腿的动静脉提取葡萄糖并没有减少。因此，经过训练的腿部，腿部葡萄糖清除率有所增加（P < 0.05），这超出了肌肉质量增加的解释。力量训练增加了 GLUT4、胰岛素受体、蛋白激酶 B-α/β、糖原合酶 (GS) 和 GS 总活性的蛋白质含量。总之，我们发现每周 3 次每次 30 分钟的力量训练可以增加两组骨骼肌中的胰岛素作用。这种适应可归因于局部收缩介导的机制，涉及胰岛素信号级联中的关键蛋白。
Strength training represents an alternative to endurance training for patients with type 2 diabetes. Little is known about the effect on insulin action and key proteins in skeletal muscle, and the necessary volume of strength training is unknown. A total of 10 type 2 diabetic subjects and 7 healthy men (control subjects) strength-trained one leg three times per week for 6 weeks while the other leg remained untrained. Each session lasted no more than 30 min. After strength training, muscle biopsies were obtained, and an isoglycemic-hyperinsulinemic clamp combined with arterio-femoral venous catheterization of both legs was carried out. In general, qualitatively similar responses were obtained in both groups. During the clamp, leg blood flow was higher (P < 0.05) in trained versus untrained legs, but despite this, arterio-venous extraction glucose did not decrease in trained legs. Thus, leg glucose clearance was increased in trained legs (P < 0.05) and more than explained by increases in muscle mass. Strength training increased protein content of GLUT4, insulin receptor, protein kinase B-alpha/beta, glycogen synthase (GS), and GS total activity. In conclusion, we found that strength training for 30 min three times per week increases insulin action in skeletal muscle in both groups. The adaptation is attributable to local contraction-mediated mechanisms involving key proteins in the insulin signaling cascade.