Th1 and Th17 immune responses to viable Propionibacterium acnes in patients with sarcoidosis
Th1 and Th17 immune responses to viable Propionibacterium acnes in patients with sarcoidosis
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DOI：
10.1016/j.resinv.2012.07.001
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发表时间：
2012-09-01
影响因子：
3.1
通讯作者：
Eishi, Yoshinobu
中科院分区：
文献类型：
--
作者：
Furusawa, Haruhiko;Suzuki, Yoshimi;Eishi, Yoshinobu
背景：痤疮丙酸杆菌和结核分枝杆菌已成为结节病的可能致病菌。方法：将来自结节病患者和健康志愿者的外周血单个核细胞（PBMC）分别与活的痤疮丙酸杆菌（P. acnes）、卡介苗（Bacille de Calmette et Guerin，BCG）共培养。结核复合体，并与早期分泌抗原靶（ESAT）-6。RT-PCR和酶联免疫斑点法（ELISPOT）检测Th 1细胞因子的产生，RT-PCR检测IL-17 mRNA的表达。结果：痤疮丙酸杆菌刺激后，结节病患者PBMC分泌IL-2的水平明显高于对照组。类似地，用痤疮丙酸杆菌刺激后，结节病患者PBMC中IL-2和IL-12 mRNA的表达显著高于对照组PBMC。相反，结节病患者PBMC中IL-17 mRNA的表达显著低于对照组。结论：结节病的发生可能是由于Th 1/Th 17免疫应答失衡引起的，而与M.肺结核综合征(C)2012日本呼吸学会。Elsevier B. V.出版，保留所有权利。
Background: Propionibacterium acnes and Mycobacterium tuberculosis have emerged as probable candidates responsible for sarcoidosis. This study was conducted to investigate the Th1/Th17 responses elicited by these pathogens in sarcoidosis and to clarify the causative role of these pathogens.Methods: Peripheral blood mononuclear cells (PBMCs) obtained from patients with sarcoidosis and from healthy volunteers were, respectively, co-cultured with viable P. acnes, with Bacille de Calmette et Guerin (BCG) as a viable M. tuberculosis complex, and with the early secretory antigenic target (ESAT)-6. Th1 cytokine production was measured using RT-PCR and enzyme-linked immunospot (ELISPOT) assays, and interleukin (IL)-17 mRNA expression was measured by RT-PCR.Results: IL-2 secretion from PBMCs after stimulation with P. acnes was significantly higher in patients with sarcoidosis than in the controls. Similarly, IL-2 and IL-12 mRNA expression after stimulation with P. acnes was significantly higher in PBMCs from patients with sarcoidosis than in PBMCs from controls. In contrast, IL-17 mRNA expression was significantly lower in PBMCs from patients with sarcoidosis than in PBMCs from controls. No significant differences between the groups were observed in the responses to stimulation with BCG or ESAT-6.Conclusion: Sarcoidosis may arise from an imbalance of Th1/Th17 immune responses against viable P. acnes, but not M. tuberculosis complex. (C) 2012 The Japanese Respiratory Society. Published by Elsevier B.V. All rights reserved.