DISTRIBUTION OF MOLECULAR AGGREGATION BY ANALYSIS OF FLUCTUATION MOMENTS
DISTRIBUTION OF MOLECULAR AGGREGATION BY ANALYSIS OF FLUCTUATION MOMENTS
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DOI：
10.1073/pnas.87.14.5479
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发表时间：
1990-07-01
影响因子：
11.1
通讯作者：
ELSON, EL
中科院分区：
文献类型：
--
作者：
QIAN, H;ELSON, EL
来自荧光分子溶液的开放体积的荧光随着分子随机地扩散到该体积中和扩散出该体积而波动。通过测量这些波动的矩或幅度分布，可以在不干扰系统的情况下表征荧光分子聚集度或聚合度的分布。我们提出了一个实验验证这种方法适用于简单的模型系统组成的解决方案的荧光颗粒的定义明确的大小。我们还表征了光子检测装置（通常是光电倍增管）的响应，这对于荧光波动的分析是必不可少的，并且比较了两种用于确定散粒噪声贡献的方法。
The fluorescence from an open volume of a solution of fluorescent molecules fluctuates as the molecules randomly diffuse into and out of the volume. The distribution of degrees of aggregation or polymerization of the fluorescent molecules can be characterized without perturbing the system by measuring either the moments or the amplitude distribution of these fluctuations. We present an experimental verification of this approach applied to simple model systems consisting of solutions of fluorescent particles of well-defined size. We have also characterized the response of the photon-detection device (typically a photomultiplier), which is essential to the analysis of the fluorescence fluctuations, and have compared two methods for determining shot-noise contributions.