Development, Validation, and Application of a Novel Method for the Analysis of Vitamin E Acetate and Other Tocopherols in Aerosol Emissions of E-Cigarettes, or Vaping Products Associated With Lung Injury.
Development, Validation, and Application of a Novel Method for the Analysis of Vitamin E Acetate and Other Tocopherols in Aerosol Emissions of E-Cigarettes, or Vaping Products Associated With Lung Injury.
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DOI：
10.3389/fchem.2021.730954
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发表时间：
2021
影响因子：
5.5
通讯作者：
Valentín-Blasini L
中科院分区：
文献类型：
--
作者：
Puetz A;Morel Espinosa M;Watson C;Blount BC;Valentín-Blasini L
在过去的4年里，电子烟或电子烟产品（EVP）的使用在美国急剧增加，特别是在年轻人和年轻人中。关于这些产品的化学成分的资料很少。通常，EVP含有溶解在促进气溶胶产生的合适溶剂中的活性成分，例如尼古丁、CBD或THC。一种EVP溶剂，维生素E醋酸酯（VEA），已在与肺损伤相关的EVP液体中进行了测量。然而，以前没有经过验证的用于测量来自这些装置的气溶胶中VEA的分析方法。因此，我们开发了一种高通量同位素稀释LC-MS/MS方法来同时测量雾化EVP样品中的VEA和其他三种相关的生育酚。含量测定具有精密度，VEA重复性范围为4.0 - 8.3%，中间精密度范围为2.5 - 6.7%。测量的其他三种生育酚获得了相似的精密度。4种分析物的检出限范围为8.85 × 10−6 ~ 2.28 × 10−5 μg/mL气雾剂喷量，校正曲线呈线性（R2> 0.99）。该方法用于分析与EVALI病例患者相关的147例EVP的气溶胶排放。我们在46%的病例相关EVP中检测到VEA，范围为1.87 × 10−4-74.1 µg/mL气雾剂喷量，平均值为25.1 µg/mL气雾剂喷量。在尼古丁或大麻二酚（CBD）产品中未检测到大量VEA（>0.1% w/w总气溶胶颗粒物）;相反，71%的EVALI相关四氢大麻酚（THC）产品含有大量VEA。在较低速率和浓度下检测到痕量水平的其他生育酚亚型（α-生育酚：检出41%，平均0.095 µg分析物/mL气雾剂喷量; γ-生育酚：检出5%，平均0.0193 µg分析物/mL气雾剂喷量; δ-生育酚：未检出）。我们的研究结果表明，VEA可以有效地转移到气雾剂EVALI相关的EVP使用标准化的协议蒸发。
E-cigarette, or vaping, product (EVP) use has increased dramatically in the United States over the last 4 years, particularly in youth and young adults. Little information is available on the chemical contents of these products. Typically, EVPs contain an active ingredient such as nicotine, CBD, or THC dissolved in a suitable solvent that facilitates aerosol generation. One EVP solvent, vitamin E acetate (VEA), has been measured in EVP liquids associated with lung injury. However, no validated analytical methods for measuring VEA in the aerosol from these devices was previously available. Therefore, we developed a high throughput isotope dilution LC-MS/MS method to simultaneously measure VEA and three other related tocopherols in aerosolized EVP samples. The assay was precise, with VEA repeatability ranging from 4.0 to 8.3% and intermediate precision ranging from 2.5 to 6.7%. Similar precision was obtained for the three other tocopherols measured. The LODs for the four analytes ranged from 8.85 × 10−6 to 2.28 × 10−5 μg analyte per mL of aerosol puff volume, and calibration curves were linear (R 2 > 0.99). This method was used to analyze aerosol emissions of 147 EVPs associated with EVALI case patients. We detected VEA in 46% of the case-associated EVPs with a range of 1.87 × 10−4–74.1 µg per mL of aerosol puff volume and mean of 25.1 µg per mL of aerosol puff volume. Macro-levels of VEA (>0.1% w/w total aerosol particulate matter) were not detected in nicotine or cannabidiol (CBD) products; conversely 71% of the EVALI associated tetrahydrocannabinol (THC) products contained macro-levels of VEA. Trace levels of other tocopherol isoforms were detected at lower rates and concentrations (α-tocopherol: 41% detected, mean 0.095 µg analyte per mL of aerosol puff volume; γ-tocopherol: 5% detected, mean 0.0193 µg analyte per mL of aerosol puff volume; δ-tocopherol: not detected). Our results indicate that VEA can be efficiently transferred to aerosol by EVALI-associated EVPs vaped using a standardized protocol.
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影响因子：
33.9
作者：
Blount, Benjamin C.;Karwowski, Mateusz P.;Pirkle, James L.
通讯作者：
Pirkle, James L.
DOI：
10.1165/rcmb.2020-0209oc
发表时间：
2020-12-01
影响因子：
6.4
作者：
Matsumoto, Shotaro;Fang, Xiaohui;Gotts, Jeffrey E.
通讯作者：
Gotts, Jeffrey E.
影响因子：
33.9
作者：
Moritz, Erin D.;Zapata, Lauren B.;Yousaf, Anna
通讯作者：
Yousaf, Anna
影响因子：
4.1
作者：
Lanina, Svetlana A.;Toledo, Patricia;Jastrebova, Jelena A.
通讯作者：
Jastrebova, Jelena A.
影响因子：
3.4
作者：
Kamal, Md Arif;Raghunathan, V. A.
通讯作者：
Raghunathan, V. A.