A Comparative Study on Asymmetric Reduction of Ketones Using the Growing and Resting Cells of Marine-Derived Fungi.
A Comparative Study on Asymmetric Reduction of Ketones Using the Growing and Resting Cells of Marine-Derived Fungi.
复制标题
利用海洋来源真菌的生长和静息细胞进行酮的不对称还原的比较研究
DOI：
10.3390/md16020062
复制
发表时间：
2018-02-14
期刊：
影响因子：
5.4
通讯作者：
Liu L
中科院分区：
文献类型：
--
作者：
Liu H;Chen BS;de Souza FZR;Liu L
全细胞生物催化剂为光学活性醇提供了一种高度对映选择性、污染最小的途径。目前，大多数全细胞催化性能涉及静息细胞而不是生长细胞生物转化，这是一种一步过程，受益于同时生长和生物转化，无需制备催化剂。本文利用海洋来源真菌的生长和休眠细胞在优化条件下成功地将 14 种芳香酮不对称还原为相应的对映体纯醇。两种方法均获得了良好的产率和优异的对映选择性。尽管底物抑制可能是生长细胞生物转化的限制因素，但所选菌株仍然可以将 10 mM 底物完全转化为所需产物。静息细胞生物转化显示出可循环九次的能力，且活性不会显着下降。这是第一项通过一步生长细胞生物转化进行酮的不对称还原的研究。
Whole-cell biocatalysts offer a highly enantioselective, minimally polluting route to optically active alcohols. Currently, most of the whole-cell catalytic performance involves resting cells rather than growing cell biotransformation, which is one-step process that benefits from the simultaneous growth and biotransformation, eliminating the need for catalysts preparation. In this paper, asymmetric reduction of 14 aromatic ketones to the corresponding enantiomerically pure alcohols was successfully conducted using the growing and resting cells of marine-derived fungi under optimized conditions. Good yields and excellent enantioselectivities were achieved with both methods. Although substrate inhibition might be a limiting factor for growing cell biotransformation, the selected strain can still completely convert 10-mM substrates into the desired products. The resting cell biotransformation showed a capacity to be recycled nine times without a significant decrease in the activity. This is the first study to perform asymmetric reduction of ketones by one-step growing cell biotransformation.
登录
查看更多内容
影响因子：
9.8
作者：
Ma, Steven K.;Gruber, John;Sheldon, Roger A.
通讯作者：
Sheldon, Roger A.
影响因子：
--
作者：
Barbieri, C;Caruso, E;Servi, S
通讯作者：
Servi, S
影响因子：
3.8
作者：
Goldberg, Katja;Edegger, Klaus;Liese, Andreas
通讯作者：
Liese, Andreas
影响因子：
3
作者：
Chen, Bi-Shuang;Liu, Hui;Liu, Lan
通讯作者：
Liu, Lan
影响因子：
3
作者：
De Vitis, Valerio;Guidi, Benedetta;Romano, Diego
通讯作者：
Romano, Diego