Characterization of environmentally friendly nicotine degradation by Pseudomonas putida biotype A strain S16
Characterization of environmentally friendly nicotine degradation by Pseudomonas putida biotype A strain S16
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恶臭假单胞菌生物型 A 菌株 S16 环境友好型尼古丁降解的表征
DOI：
10.1099/mic.0.2006/005223-0
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发表时间：
2007-05-01
期刊：
影响因子：
2.8
通讯作者：
Xu, Ping
中科院分区：
文献类型：
--
作者：
Wang, Shu Ning;Liu, Zhen;Xu, Ping
烟草和烟草废料中的尼古丁和一些相关生物碱对健康和环境有害，主要的环境要求是从烟草和烟草废料中去除它们。在这项研究中，一个分离的菌株，S16，鉴定为恶臭假单胞菌生物型A，用于研究尼古丁的降解。根据NMR、傅立叶变换（FT）-IR和UV光谱、GC-MS和高分辨率MS（HR-MS）分析结果鉴定了可能的中间体。在恶臭假单胞菌中，尼古丁的降解途径为通过中间体N-甲基麦斯明、2 '-羟基烟碱、假氧化尼古丁、3-吡啶丁醛、C-氧代、3-琥珀酰基吡啶和6-羟基-3-琥珀酰基吡啶从尼古丁降解为2，5-二羟基吡啶。首次检测到N-甲基麦斯明、2，5-二羟基吡啶和琥珀酸作为尼古丁降解的中间体，并得到了令人满意的验证。此外，还发现了一种新的烟碱降解产物，即1 -丁酮，4-羟基-1-（3-吡啶基）。这些发现为尼古丁的微生物代谢和尼古丁降解的环境友好途径提供了新的见解。
Nicotine and some related alkaloids in tobacco and tobacco wastes are harmful to health and the environment, and a major environmental requirement is to remove them from tobacco and tobacco wastes. In this study, an isolated strain, S16, identified as Pseudomonas putida biotype A, was used to investigate nicotine degradation. Possible intermediates were identified based on the results of NMR, Fourier-transform (FT)-IR and UV spectroscopy, GC-MS and high-resolution MS (HR-MS) analysis. The pathway of nicotine degradation in P. putida was proposed to be from nicotine to 2,5-dihydroxypyridine through the intermediates N-methylmyosmine, 2'-hydroxynicotine, pseudooxynicotine, 3-pyridinebutanal,C-oxo, 3-succinoylpyridine and 6-hydroxy-3-succinoylpyridine. N-Methylmyosmine, 2,5-dihydroxypyridine and succinic acid were detected and satisfactorily verified for the first time as intermediates of nicotine degradation. In addition, an alcohol compound, 1 -butanone,4-hydroxy- 1 -(3-pyridinyl), was found to be a novel product of nicotine degradation. These findings provide new insights into the microbial metabolism of nicotine and the environmentally friendly route of nicotine degradation.