Nuclear pores form de novo from both sides of the nuclear envelope
Nuclear pores form de novo from both sides of the nuclear envelope
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DOI：
10.1126/science.1124196
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发表时间：
2006-04-21
期刊：
影响因子：
56.9
通讯作者：
Hetzer, MW
中科院分区：
文献类型：
--
作者：
D'Angelo, MA;Anderson, DJ;Hetzer, MW
核孔复合物是跨越核膜双脂双层的多蛋白通道。新的孔是如何插入到增殖和分化的真核细胞的完整核膜中的尚不清楚。我们发现Nup 107 -160复合物从核质和细胞质两侧被纳入核膜中的组装位点。核孔插入需要在细胞核和细胞质隔室中产生Ran鸟苷三磷酸。新形成的核孔复合物不包含预先存在的孔的结构成分，这表明它们可以从头形成。
Nuclear pore complexes are multiprotein channels that span the double lipid bilayer of the nuclear envelope. How new pores are inserted into the intact nuclear envelope of proliferating and differentiating eukaryotic cells is unknown. We found that the Nup107-160 complex was incorporated into assembly sites in the nuclear envelope from both the nucleoplasmic and the cytoplasmic sides. Nuclear pore insertion required the generation of Ran guanosine triphosphate in the nuclear and cytoplasmic compartments. Newly formed nuclear pore complexes did not contain structural components of preexisting pores, suggesting that they can form de novo.