Effect of Roucongrong (Herba Cistanches Deserticolae) decoction on the substantia nigra through Wnt/β-catenin signaling pathway in rats with Parkinson's disease induced by 6-hydroxydopamine hydrochloride
Effect of Roucongrong (Herba Cistanches Deserticolae) decoction on the substantia nigra through Wnt/β-catenin signaling pathway in rats with Parkinson's disease induced by 6-hydroxydopamine hydrochloride
复制标题
肉从荣汤通过Wnt/β-catenin信号通路对6-羟基多巴胺致帕金森病大鼠黑质的影响
DOI：
10.19852/j.cnki.jtcm.2021.05.010
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发表时间：
2021
影响因子：
2.6
通讯作者：
Cai Jing
中科院分区：
文献类型：
--
作者：
Xu Qian;Qin Wei;Wu Fangzhen;Lin Yao;Hong Liting;Chen Dan;Hu Xuefeng;Cai Jing
目的：观察肉毒蓉对6-羟基多巴胺(6-OHDA)诱导的帕金森病(PD)大鼠黑质的影响。以进一步确定Wnt/β-连环蛋白信号通路是否参与了这一作用。方法：脑内注射6-OHDA建立帕金森病大鼠模型。随后，制备三种浓度的肉毒蓉汤，灌胃给药14d，观察帕金森病大鼠的行为学变化。用免疫组织化学方法检测黑质酪氨酸羟化酶(TH)的表达。用Western blotting和聚合酶链式反应检测Wnt/β-catenin信号通路成员的基因和蛋白表达水平。最后，用WNT/β-连环蛋白抑制剂研究了1-甲基-4-苯基吡啶(MPP+)对MES23.5细胞的作用机制。结果：肉毒杆菌汤可改善帕金森病大鼠的步态和步态调整能力。它还增加了黑质中的TH，并改变了Wnt/β-连环蛋白信号通路中的基因和蛋白的表达。WNT/β-连环蛋白抑制剂可降低肉桂糖浆煎剂对MPP+诱导的MES23.5细胞的抑制作用。结论：肉苁蓉煎剂可能通过增加黑质TH含量，激活Wnt/β-Catenin信号通路，促进帕金森病大鼠体内、外神经元存活。
OBJECTIVE: To investigate the effect of Roucongrong (Herba Cistanches Deserticolae) decoction on the substantia nigra in rats with Parkinson's disease (PD) induced by 6-hydroxydopamine hydrochloride (6-OHDA). To further determine whether the Wnt/β-catenin signaling pathway is involved in the action. METHODS: A rat model of PD was established by intracranial injection of 6-OHDA. Subsequently, three concentrations of Roucongrong (Herba Cistanches Deserticolae) decoction were prepared and administered to rats by gavage therapy for 14 d. Behavioral changes were measured in PD rats. In vivo tyrosine hydroxylase (TH) levels in the substantia nigra were examined by immunohistochemistry. Additionally, gene and protein expression levels of members of the Wnt/β-catenin signaling pathway were examined by Western blotting and polymerase chain reaction. Lastly, a Wnt/β-catenin inhibitor was used to investigate the mechanism of action in 1-methyl-4-phenylpyridinium (MPP + )- treated MES23.5 cells in vitro. RESULTS: Roucongrong (Herba Cistanches Deserticolae) decoction improved performance in the stride and gait adjustment tests in PD rats. It also increased TH in the substantia nigra and altered the expression of genes and proteins in the Wnt/ β-catenin signaling pathway. Wnt/β-catenin inhibitor reduced the effect of Roucongrong (Herba Cistanches Deserticolae) decoction in MPP +-treated MES23.5 cells. CONCLUSION: Roucongrong (Herba Cistanches Deserticolae) decoction may promote neuronal survival in PD in vivo and in vitro by increasing TH content in the substantia nigra and by activating the Wnt/ β-catenin signaling pathway.