Nested retrotransposons in the intergenic regions of the maize genome
Nested retrotransposons in the intergenic regions of the maize genome
复制标题
DOI：
10.1126/science.274.5288.765
复制
发表时间：
1996-11-01
期刊：
影响因子：
56.9
通讯作者：
Bennetzen, JL
中科院分区：
文献类型：
--
作者：
SanMiguel, P;Tikhonov, A;Bennetzen, JL
复杂真核生物基因组中基因和重复DNA的相关组织情况尚未被充分了解。诊断性测序表明，包含玉米Adh1 - F和u22基因的一个280千碱基区域主要由相互插入的反转录转座子组成。发现了10个反转录元件家族，其在单倍体基因组中的重复频率从10到30000拷贝不等。这些反转录转座子占Adh1 - F区域的60%以上，并且至少占玉米核DNA的50%。这些元件大部分是完整的，并且分散在玉米基因组含基因的区域。
The relative organization of genes and repetitive DNAs in complex eukaryotic genomes is not well understood. Diagnostic sequencing indicated that a 280-kilobase region containing the maize Adh1-F and u22 genes is composed primarily of retrotransposons inserted within each other. Ten retroelement families were discovered, with reiteration frequencies ranging from 10 to 30,000 copies per haploid genome, These retrotransposons accounted for more than 60 percent of the Adh1-F region and at least 50 percent of the nuclear DNA of maize. These elements were largely intact and are dispersed throughout the gene-containing regions of the maize genome.