T47D breast cancer cell growth is inhibited by expression of VACM-1, a cul-5 gene
T47D breast cancer cell growth is inhibited by expression of VACM-1, a cul-5 gene
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DOI：
10.1016/j.bbrc.2004.05.057
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发表时间：
2004-07-02
影响因子：
3.1
通讯作者：
Barney, CC
中科院分区：
文献类型：
--
作者：
Burnatowska-Hledin, MA;Kossoris, JB;Barney, CC
抗利尿激素激活的钙动员（VACM-1）是一个cull -5基因，位于染色体11q22-23上，靠近与乳腺癌肿瘤样本中杂合性缺失相关的共济失调毛细血管扩张基因。为了研究VACM-1的生物学作用，我们研究了VACM-1表达对T47D乳腺癌细胞生长和基因表达的影响。免疫细胞化学研究表明，VACM-1在0.6-6%的T47D细胞中表达，并定位于有丝分裂细胞的细胞核。与对照细胞相比，过表达VACM-1显著减弱细胞增殖和MAPK磷酸化。此外，VACM-1降低egr-1，增加Fas-L mRNA水平。此外，与对照组相比，VACM-1转染细胞的核分数中egr-1蛋白水平显著降低。这些数据表明VACM-1参与了细胞生长的调控。(C) 2004爱思唯尔公司版权所有。
Vasopressin-activated calcium-mobilizing (VACM-1), a cul-5 gene, is localized on chromosome 11q22-23 close to the gene for Ataxia Telangiectasia in a region associated with a loss of heterozygosity in breast cancer tumor samples. To examine the biological role of VACM-1, we studied the effect of VACM-1 expression on cellular growth and gene expression in T47D breast cancer cells. Immunocytochemistry studies demonstrated that VACM-1 was expressed in 0.6-6% of the T47D cells and localized to the nucleus of mitotic cells. Overexpressing VACM-1 significantly attenuated cellular proliferation and MAPK phosphorylation when compared to the control cells. In addition, VACM-1 decreased egr-1 and increased Fas-L mRNA levels. Further, egr-1 protein levels were significantly lower in the nuclear fraction from VACM-1 transfected cells when compared to controls. These data indicate that VACM-1 is involved in the regulation of cellular growth. (C) 2004 Elsevier Inc. All rights reserved.