EXPRESSION OF CELL-SURFACE MARKERS AND MYELIN PROTEINS IN CULTURED OLIGODENDROCYTES FROM NEONATAL BRAIN OF RAT AND MOUSE - A COMPARATIVE-STUDY
EXPRESSION OF CELL-SURFACE MARKERS AND MYELIN PROTEINS IN CULTURED OLIGODENDROCYTES FROM NEONATAL BRAIN OF RAT AND MOUSE - A COMPARATIVE-STUDY
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DOI：
10.1159/000111276
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发表时间：
1995-03-01
影响因子：
2.9
通讯作者：
MACKLIN, WB
中科院分区：
文献类型：
--
作者：
DUCHALA, CS;ASOTRA, K;MACKLIN, WB
分离的脑细胞培养物是研究少突胶质细胞体外发育和分化的有用模型。目前的研究比较了来自小鼠和大鼠新生儿的原发性和继发性少突胶质细胞培养物中少突胶质细胞谱系/髓鞘标记物的表达的发育模式。大鼠和小鼠分离的脑细胞培养表达相同的髓鞘特异性抗原，但小鼠少突胶质细胞产生一个更大，更详细的片状膜比大鼠少突胶质细胞，和一些髓鞘标记物（O 4，GC，和MBP）在小鼠培养物中显示更强烈的膜染色。GD（3）似乎是大鼠细胞的一个很好的少突胶质细胞标记物，但在小鼠细胞中是非特异性的。在小鼠培养物中少突胶质细胞较少，并且它们似乎需要比大鼠少突胶质细胞更长的分化时间。在次级少突胶质细胞培养物中也观察到这些相同的结果，尽管通常晚期髓鞘标记物如MBP和PLP在小鼠细胞中的表达水平比大鼠细胞低得多。
Dissociated brain cell cultures are a useful model for investigating development and differentiation of oligodendrocytes in vitro. The current studies compare the developmental patterns of expression for oligodendrocyte lineage/myelin markers in both primary and secondary oligodendrocyte cultures derived from mouse and rat neonates. The rat and mouse dissociated brain cell cultures express the same myelin-specific antigens, but mouse oligodendrocytes produce a larger and more elaborate sheet-like membrane than rat oligodendrocytes, and some of the myelin markers (O4, GC, and MBP) show more intense membrane staining in mouse cultures. GD(3) appears to be a good oligodendrocyte marker for rat cells, but it is nonspecific in mouse cells. There are fewer oligodendrocytes in mouse cultures, and they appear to require a longer differentiation time than rat oligodendrocytes. These same results are also observed in secondary oligodendrocyte cultures, although in general late myelin markers such as MBP and PLP are expressed at a much lower level in mouse cells than rat cells.