A study of oxidative stress induced by two polybrominated diphenyl ethers in the rotifer Brachionus plicatilis.
A study of oxidative stress induced by two polybrominated diphenyl ethers in the rotifer Brachionus plicatilis.
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DOI：
10.1016/j.marpolbul.2016.10.032
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发表时间：
2016-12
影响因子：
5.8
通讯作者：
Jing Zhang;You Wang;Kai-Ming Sun;K. Fang;Xue-Xi Tang
中科院分区：
文献类型：
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作者：
Jing Zhang;You Wang;Kai-Ming Sun;K. Fang;Xue-Xi Tang
多溴联苯醚(PBDEs)是海洋生态系统中广泛分布的持久性有机污染物。然而，它们对海洋生物，特别是无脊椎动物的毒性机制仍然知之甚少。研究了多溴联苯醚的两种常见同系物：四溴联苯醚-47(BDE-47)和十溴联苯醚-209(BDE-209)。它们的毒性机制，重点是氧化应激，在褶皱臂尾轮虫身上进行了研究。两种多溴二苯醚均可诱导细胞产生过量的ROS。超氧化物歧化酶(SOD)基因表达增加，提示SOD在清除ROS中起主要作用。细胞内钙离子浓度和钙调素(CaM)基因表达增加。这表明钙离子(Ca~(2+))信号通道参与了PBDEs应激。进一步分析表明，生殖系统可能是多溴二苯醚毒性的靶点。此外，BDE-47试验组检测指标的高值表明，BDE-47可能比BDE-209对轮虫造成更高的氧化损伤。
Polybrominated diphenyl ethers (PBDEs) are widely dispersed persistent organic pollutants in the marine ecosystem. However, their toxic mechanisms in marine organisms, especially invertebrates, remain poorly understood. Two common congeners of PBDEs, tetrabrominated diphenyl ether-47 (BDE-47) and decabrominated diphenyl ether-209 (BDE-209), were investigated. Their toxic mechanisms, with a focus on oxidative stress, were examined in rotifer Brachionus plicatilis. Overproduction of reactive oxygen species (ROS) was induced by two PBDEs. The expression of superoxide dismutase (SOD) mRNA was increased, suggesting SOD play a main role in ROS-scavenging. The intercellular concentrations of calcium ([Ca2 +]in) and the expression of calmodulin (CaM) mRNA were increased. This indicates the calcium ion (Ca2 +) signaling channel is involved in PBDEs stress. Further analysis showed that the reproductive system might be the target site for toxicity of PBDEs. Moreover, high value of detection indexes in BDE-47 experimental groups suggested BDE-47 might cause higher oxidative damage than BDE-209 in rotifers.