Sequential Exposure to Obesogenic Factors in Females Rats: From Physiological Changes to Lipid Metabolism in Liver and Mesenteric Adipose Tissue.
Sequential Exposure to Obesogenic Factors in Females Rats: From Physiological Changes to Lipid Metabolism in Liver and Mesenteric Adipose Tissue.
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DOI：
10.1038/srep46194
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发表时间：
2017-04-07
影响因子：
4.6
通讯作者：
Diéguez C
中科院分区：
文献类型：
--
作者：
Novelle MG;Vázquez MJ;Peinado JR;Martinello KD;López M;Luckman SM;Tena-Sempere M;Malagón MM;Nogueiras R;Diéguez C
在她们的一生中，女性受到不同的营养和激素因素的影响，这些因素可能会增加肥胖和相关合并症的风险。从产后早期到绝经后，暴露于营养过剩、高能量饮食和雌激素缺乏被认为是妇女肥胖的重要危险因素。在这项研究中，我们评估了关键的过渡生活事件和暴露于不同的营养如何影响大鼠模型的能量稳态。具体来说，我们评估了在断奶后连续暴露于产后过度营养、高脂肪饮食（HFD），随后进行卵巢切除术（OVX；作为绝经模型）。每个肥胖危险因素显著增加体重（BW）和肥胖，在连续暴露后具有累加效应。HFD和/或OVX组的能量摄入增加，OVX后运动活动和能量消耗减少可以解释这些代谢变化。我们的研究还记录了HFD和/或OVX后肠系膜脂肪组织的脂肪生成途径减少，独立于先前的产后规划，但只有HFD在肝脏中引起了这种影响。此外，我们报告了肝脏PEPCK表达的增加取决于先前的代谢状态。总的来说，我们的研究结果确定了不同风险因素的影响，这将有助于理解女性肥胖的发展。
During their lifetime, females are subjected to different nutritional and hormonal factors that could increase the risk of obesity and associated comorbidities. From early postnatal periods until the postmenopausal phase, exposure to over nutrition, high-energy diet and oestrogen deficiency, are considered as significant obesity risk factors in women. In this study, we assessed how key transitional life events and exposure to different nutrition influence energy homeostasis in a rat model. Specifically, we assessed the sequential exposure to postnatal over nutrition, high-fat diet (HFD) after weaning, followed later by ovariectomy (OVX; as a model of menopause). Each obesity risk factor increased significantly body weight (BW) and adiposity, with additive effects after sequential exposure. Increased energy intake in both HFD and/or OVX groups, and decreased locomotor activity and energy expenditure after OVX can explain these metabolic changes. Our study also documents decreased lipogenic pathway in mesenteric adipose tissue after HFD and/or OVX, independent of previous postnatal programming, yet only HFD evoked this effect in liver. In addition, we report an increase in the expression of the hepatic PEPCK depending on previous metabolic status. Overall, our results identify the impact of different risk factors, which will help in understanding the development of obesity in females.