Effects of icariin on the osteogenic differentiation of bone marrow mysenchymal stem cells in vitro
Effects of icariin on the osteogenic differentiation of bone marrow mysenchymal stem cells in vitro
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发表时间：
2008
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通讯作者：
Ma Huiping
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作者：
Ma Huiping
目的研究淫羊藿苷对骨髓间充质干细胞成骨分化的影响。将10-5和10-4 mol/L淫羊藿苷分别加入大鼠骨髓间充质干细胞（bone marrow mysenchymal stem cells，rBMSCs）原代培养液中，MTT法检测细胞增殖情况，并进行比较，观察成骨分化标志物CFU-F_（10 - 5）和CFU-F_（10- 4）对rBMSCs成骨分化的影响。ALP、碱性磷酸酶（ALP）活性、骨钙素分泌，结果淫羊藿苷对原代培养的rBMSCs增殖无刺激作用，但对增殖有抑制作用，10 -5 mol/L淫羊藿苷对rBMSCs增殖有抑制作用，而对骨钙沉积和矿化骨模量无明显影响，对骨相关蛋白和细胞因子的基因表达有抑制作用。淫羊藿苷能显著促进rBMSCs的成骨分化，表现为CFU-F_ALP数量、ALP活性、骨钙素分泌、与对照组相比，补充淫羊藿苷组的钙沉积和矿化模量。结论淫羊藿苷对BMSCs的成骨分化有较强的促进作用，提示淫羊藿苷具有增强成骨活性的作用。
Objective To investigate the effects of icariin on the osteogenic differentiation of bone marrow mysenchymal stem cells.Methods 10-8,10-7,10-6,10-5 and 10-4 mol/L icariin were supplemented into the culture media of primary rat bone marrow mysenchymal stem cells(rBMSCs) separately.The cell proliferation was assayed by MTT method and compared with each other.The osteogenic differentiation markers including CFU-F_ALP,alkaline phosphatase(ALP) activity,osteocalcin secretion,calcium deposition and mineralized bone modulus were compared between the icariin-supplemented group and the control.The gene expression of bone-related protein and cytokines were analysed by common RT-PCR and real-time RT-PCR.Results Icariin didn't stimulate but inhibit the proliferation of rBMSCs at primary culture.10-5 mol/L icariin strongly enhances the osteogenic differentiation of rBMSCs which was shown by significantly improved CFU-F_ALP number,ALP activity,osteocalcin secretion,calcium deposition and mineralized modulus in the icarrin-supplemented group compared with the control.The gene expression level of bone-related marker proteins and cytokines were also obviously higher in the former than in the latter group.Conclusion Icariin strongly stimulates the osteogenic differentiation of BMSCs,which indicates that icariin has the ability to enhance the bone formation activity.