Localization of Δ4-5β and 5α-Reductases and 17β-ol-Dehydrogenase in Immature Golden Hamster Testes*
Localization of Δ4-5β and 5α-Reductases and 17β-ol-Dehydrogenase in Immature Golden Hamster Testes*
复制标题
未成熟金仓鼠睾丸中 Δ4-5β 和 5α-还原酶以及 17β-ol-脱氢酶的定位*
DOI：
10.1210/endo-111-1-269
复制
发表时间：
1982
期刊：
影响因子：
4.8
通讯作者：
Keishi Matsumoto
中科院分区：
文献类型：
--
作者：
Y. Furubayashi;N. Terada;B. Sato;Keishi Matsumoto
用湿法将28日龄和70日龄金黄地鼠的睾丸分离为输精小管和间质组织。组织匀浆和亚细胞部分与[14C]雄烯二酮孵育，计算NADPH和酶活性(每100 mg蛋白/小时纳米分子)。在未成熟的睾丸中，5-β-还原酶主要定位于曲细精管，而5-α-还原酶定位于间质组织。17β-OLD脱氢酶活性在肾小管和间质中分布均匀。虽然5个β-和5个α-还原酶的活性在未成熟的睾丸中约为成人睾丸的10倍，但在成人睾丸中的17β-ol-脱氢酶活性约为未成熟睾丸的10倍。在未成熟的精巢中，5-α-还原酶和17-β-ol-脱氢酶大部分定位于微粒体，而5-β-还原酶全部定位于胞浆。这些结果表明，β4-3-酮类固醇类化合物发生了较大的5-Δ还原反应.
Testes of 28- and 70-day-old golden hamsters were separated into seminiferus tubules and interstitial tissue by wet dissection. Homogenates of the tissues and subcellular fractions were incubated with [14C]androstenedione, and NADPH and enzyme activity (nanomoles per 100 mg protein/h) was estimated. In immature testes, the 5β-reductase activity was largely confined to the seminiferous tubules, while 5α-reductase was localized in the interstitial tissue. The activity of 17β-oldehydrogenase was found to be equally distributed between tubules and interstitial tissue. Although the activities of 5β- and 5α-reductases were approximately 10 times greater in immature than adult testes, the 17β-ol-dehydrogenase activity in adult testes was approximately 10 times that in immature testes. In the immature testes, most 5α-reductase and 17β-ol-dehydrogenase were localized in microsomes, whereas 5β-reductase was wholly localized in cytosol. These results indicate that 5β-reduction of Δ4-3-ketosteroids takes place largel...