Sources of Salmonella contamination during broiler production in Eastern Spain
Sources of Salmonella contamination during broiler production in Eastern Spain
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DOI：
10.1016/j.prevetmed.2010.09.006
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发表时间：
2011-01-01
影响因子：
2.6
通讯作者：
Lainez, M.
中科院分区：
文献类型：
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作者：
Marin, C.;Balasch, S.;Lainez, M.
预防家禽产品沙门氏菌污染需要详细了解其在生产系统中存在的主要来源。本研究的目的是确定生长过程中肉鸡生产中沙门氏菌污染的主要来源，评估饲养期结束时沙门氏菌污染的风险因素，并确定西班牙东部肉鸡生产系统中涉及的主要血清型。对来自不同农场的65个不同肉鸡舍进行了抽样。在饲养期间的不同时间对每个房屋进行采样。首先，当前一个羊群被带到屠宰场时，收集灰尘、表面和前一个羊群粪便的样本。清洁和消毒（C&D）后，还采集了灰尘和表面样本。在饲养的第一天，收集了水、垫料、农靴、粪、运输箱衬垫和饲料的样品。在饲养过程中，饲料样品直接从卡车和喂食器中采集。在屠宰当天，还收集了灰尘、表面、水、饲料和粪便的样本。最后，屠宰后两天，携带者（啮齿动物，苍蝇和甲虫）被捕获。根据ISO 6579：2002（附录D）分析采集的所有样本，并根据Kauffman-White-Le-Minor技术对阳性样本进行血清分型。我们的研究结果表明，所有不同类型的样本收集沙门氏菌污染（患病率范围在1.5%和38.6%之间）。与家禽生产相关的污染最严重的样本是：送货箱内衬（32.0%）、粪便样本（31.2%）、灰尘样本（25.0%）、农用靴（19.7%）和饲养员的饲料（16.0%）。然而，在饲养期结束时鸡群沙门氏菌污染的最重要的风险因素是清洁和消毒后房屋的沙门氏菌状态，日龄鸡群的沙门氏菌状态和饲养员的饲料。从分析的样品中分离出21种不同的血清型。流行程度依次为肠道沙门氏菌（52.9%）、沙门氏菌（52.9%）、沙门氏菌（52.9%）、沙门氏菌（52.9%）、沙门氏菌（52.9%）。Hadar（17.8%）、S. Virchow（8.9%）和S.俄亥俄州（5.4%）。研究表明，沙门氏菌污染的来源很多，在肉鸡生产中具有持久性。因此，需要控制整个生产链，以消除初级生产中的细菌。(C)2010 Elsevier B. V.保留所有权利。
Prevention of Salmonella contamination of poultry products requires detailed knowledge of the main sources associated with its presence in the production system. The aims of this study were to determine the main sources of Salmonella contamination in broiler production during growing, to assess the risk factors for Salmonella contamination at the end of the rearing period and to determine the main serovars involved in broiler production systems in Eastern Spain. A total of 65 different broiler houses from different farms were sampled. Each house was sampled at different times during the rearing period. First, when the previous flock was taken to the slaughterhouse, samples of dust, surfaces and previous flock faeces were collected. After cleaning and disinfection (C&D), samples of dust and surfaces were also taken. On the first day of rearing, samples of water, bedding, farming boots, meconiums, delivery-box liners and feed were collected. During rearing, feed samples were taken directly from the truck and from feeders. On slaughter day, samples of dust, surfaces, water, feed and faeces were also collected. Finally, two days after slaughter, carriers (rodents, flies and beetles) were trapped. All samples collected were analysed according to ISO 6579:2002 (Annex D) and positive samples were serotyped in accordance with Kauffman-White-Le-Minor technique. Our results showed that all different types of samples collected were contaminated with Salmonella (prevalence ranged between 1.5% and 38.6%). The most contaminated samples related with poultry production were: delivery-box liners (32.0%), faeces samples (31.2%), dust samples (25.0%), farming boots (19.7%) and feed from feeders (16.0%). However, the most important risk factors for Salmonella contamination of the flocks at the end of the rearing period were Salmonella status of the house after cleaning and disinfection, Salmonella status of day-old chick flocks and feed from feeders. Twenty-one different serovars were isolated from the samples analysed. The most prevalent were in decreasing order: Salmonella Enteritidis (52.9%), S. Hadar (17.8%), S. Virchow (8.9%) and S. Ohio (5.4%). The study suggested that there are many sources for Salmonella contamination and persistence in broiler production. Hence, the whole production chain needs to be controlled to eradicate the bacteria from primary production. (C) 2010 Elsevier B.V. All rights reserved.