Cbx2, a PcG Family Gene, Plays a Regulatory Role in Medaka Gonadal Development
Cbx2, a PcG Family Gene, Plays a Regulatory Role in Medaka Gonadal Development
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DOI：
10.3390/ijms21041288
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发表时间：
2020-02-01
影响因子：
5.6
通讯作者：
Zhang, Junling
中科院分区：
文献类型：
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作者：
Chao, Qinghe;Shen, Fengfeng;Zhang, Junling
染色体盒同源物2（CBX 2）是多梳组（PcG）家族的重要成员，在哺乳动物性腺发育中起重要作用。cbx2的功能缺陷或基因突变可导致小鼠和人类的性别逆转。然而，关于cbx2在鱼类性腺发育中的功能知之甚少。在这项研究中，cbx2基因被确定在青鳉，这是一个模式物种的研究性腺发育的鱼类。cbx2的转录在性腺中是丰富的，睾丸水平相对高于卵巢水平。原位杂交（ISH）显示，cbx 2 mRNA主要定位于精原细胞和精母细胞，并观察到在卵母细胞在第I，II和III阶段。此外，cbx2和vasa（标记基因）共定位在生殖细胞的荧光原位杂交（FISH）。在性腺中通过RNA干扰（RNAi）敲低cbx2后，性别相关基因（包括sox9和foxl2）受到影响。这些结果表明，cbx2不仅在精子发生和卵子发生中发挥积极作用，而且还通过调节性别相关基因的表达水平参与性腺分化。
Chromobox homolog 2 (CBX2), a key member of the polycomb group (PcG) family, is essential for gonadal development in mammals. A functional deficiency or genetic mutation in cbx2 can lead to sex reversal in mice and humans. However, little is known about the function of cbx2 in gonadal development in fish. In this study, the cbx2 gene was identified in medaka, which is a model species for the study of gonadal development in fish. Transcription of cbx2 was abundant in the gonads, with testicular levels relatively higher than ovarian levels. In situ hybridization (ISH) revealed that cbx2 mRNA was predominately localized in spermatogonia and spermatocytes, and was also observed in oocytes at stages I, II, and III. Furthermore, cbx2 and vasa (a marker gene) were co-localized in germ cells by fluorescent in situ hybridization (FISH). After cbx2 knockdown in the gonads by RNA interference (RNAi), the sex-related genes, including sox9 and foxl2, were influenced. These results suggest that cbx2 not only plays a positive role in spermatogenesis and oogenesis but is also involved in gonadal differentiation through regulating the expression levels of sex-related genes in fish.