Establishment of cell suspension culture of Lonicera japonica Thunb and analysis its major secondary metabolites
Establishment of cell suspension culture of Lonicera japonica Thunb and analysis its major secondary metabolites
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金银花细胞悬浮培养物的建立及其主要次生代谢产物分析
DOI：
10.1016/j.indcrop.2019.05.024
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发表时间：
2019-10-01
影响因子：
5.9
通讯作者：
Xiong, Jianhua
中科院分区：
文献类型：
--
作者：
Hu, Min;Hu, Zhaojun;Xiong, Jianhua
金银花是原产于亚洲（包括中国，韩国和日本）的金银花，由于其广泛的生物活性和药用特性，经常用于健身。本研究的目的是建立L.以及鉴定和定量其主要次生代谢产物。灭菌后，L.在MS + NAA1.5mgL-1，2，4-D1.0mgL-1，KIN0.75mgL-1，BA0.15mgL-1，BA的培养基上，可诱导出黄色、易碎的愈伤组织。将诱导的愈伤组织转移到MS + BA 1.5 mg L ~（-1）+ NAA 0.2 mg L ~（-1）+ 2，4 D 0.1 mg L ~（-1）的培养基上，继代3 ~ 4次，获得均一的愈伤组织系。成功建立了细胞悬浮培养体系，其生长曲线呈“S”型，细胞形态健康。悬浮培养细胞中主要次生代谢产物为3，5-二-O-咖啡酰奎宁酸、3-O-咖啡酰奎宁酸、4，5-二-O-咖啡酰奎宁酸和3，4-二-O-咖啡酰奎宁酸。悬浮细胞中绿原酸的总含量为22.7mg·g ~（-1）。
Lonicera japonica Thunb, as a species of honeysuckle native to Asia (including China, Korea, and Japan), is often used for physical fitness due to its extensive biological activity and pharmaceutical properties. The goal of this work was to establish the cell suspension culture of L. japonica as well as to identify and quantify its major secondary metabolites. After sterilized, L. japonica buds, the best explant compared with young stems and leave, can be induced to form yellow friable callus on the MS medium supplemented with 1.5 mg L-1 NAA, 1.0 mg L-1 2,4D, 0.75 mg L-1 KIN and 0.15 mg L-1 BA. The induced callus was transferred on MS medium containing BA 1.5 mg L-1 + NAA 0.2 mg L-1 + 2,4D 0.1 mg L-1, and then sub-cultured three or four times to generate homogenous callus line. A cell suspension system was successfully established, with characteristic of a "S" growth curve and healthy morphology. 3,5-di-O-caffeoylquinic acid, 3-O-caffeoylquinic acid, 4,5-di-O-caffeoylquinic acid and 3,4-di-O-caffeoylquinic acid were the main secondary metabolites in the suspension culture cells. The total content of these chlorogenic acids was 22.7 mg g(-1) in the suspension cells.