Efficient extraction of low-abundance peptides from digested proteins and simultaneous exclusion of large-sized proteins with novel hydrophilic magnetic zeolitic imidazolate frameworks
Efficient extraction of low-abundance peptides from digested proteins and simultaneous exclusion of large-sized proteins with novel hydrophilic magnetic zeolitic imidazolate frameworks
复制标题
利用新型亲水性磁性沸石咪唑酯框架，从消化的蛋白质中高效提取低丰度肽，并同时排除大尺寸蛋白质
DOI：
10.1016/j.talanta.2017.02.038
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发表时间：
2017
期刊：
影响因子：
6.1
通讯作者：
Deng Chunhui
中科院分区：
文献类型：
--
作者：
Zhao Man;Xie Yiqin;Chen Hemei;Deng Chunhui
咪唑类沸石骨架是由四面体过渡金属离子和咪唑类有机连接基团组成的。这种金属有机骨架（MOFs）结合了沸石和MOFs的独特性质。此外，ZIF可以在温和的生物相容性条件下组装。这些优点使其主要用于生化分析。本研究以一种简单温和的方法合成了聚多巴胺修饰的亲水性磁性ZIF，用于低丰度多肽的高效提取。由于低配位的Zn 2+离子，Fe3O4@PDA@ZIF-8复合物对低丰度肽，特别是那些具有组氨酸残基的肽显示出强的亲和力。富集后，BSA和HSA胰蛋白酶消化物的序列覆盖率得到了显著提高。基于磁性ZIF的固相方法对大尺寸蛋白质表现出尺寸排阻效应，即使在用BSA蛋白质溶液稀释200倍的情况下也被证明是有效的。
Zeolitic imidazolate frameworks (ZIFs) are composed of tetrahedral transition metal ions and imidazolate type organic linkers. This kind of metal organic frameworks (MOFs) combines the unique properties of both zeolites and MOFs. Furthermore, ZIFs can be assembled under mild biocompatible conditions. These advantages make them be primarily studied for biochemical analysis. In this work, polydopamine-modified hydrophilic magnetic ZIFs were synthesized in a simple and mild way for the efficient extraction of low-abundance peptides. Thanks to the low-coordinated Zn2+ions, the Fe3O4@PDA@ZIF-8 composites showed strong affinity towards low-abundance peptides, especially those with histidine residues. After enrichment, the sequence coverage of BSA and HSA tryptic digest got a significant increase. The solid phase method based on the magnetic ZIFs exhibited size-exclusion effect towards large-sized proteins, and proved to be effective even under the 200-fold dilution with BSA protein solution.