High mannose type N-linked oligosaccharides on endothelial cells may influence beta 2 integrin mediated neutrophil adherence in vitro.
High mannose type N-linked oligosaccharides on endothelial cells may influence beta 2 integrin mediated neutrophil adherence in vitro.
复制标题
内皮细胞上的高甘露糖型 N 连接寡糖可能会影响体外 β2 整合素介导的中性粒细胞粘附。
DOI：
10.1002/jcb.240510316
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发表时间：
1993
影响因子：
4
通讯作者：
Gehlsen,KR
中科院分区：
文献类型：
--
作者：
Sriramarao,P;Berger,E;Chambers,JD;Arfors,KE;Gehlsen,KR
我们在此报告了内皮细胞表面蛋白的N-连接寡糖加工对中性粒细胞粘附的作用。人脐静脉内皮细胞单层处理24小时，与脱氧mannojirimycin（DMJ），高尔基甘露糖苷酶I的抑制剂，导致糖蛋白加工的变化，然后与中性粒细胞孵育，以检查他们的能力，坚持处理的内皮细胞。用DMJ处理，导致高甘露糖型寡糖的积累，导致佛波酯（PMA）活化的中性粒细胞的粘附性比附着于未处理的内皮细胞增加两倍。这种粘附可能由β2整合素Mac-1介导，并且可以用ICAM-1和整合素β2亚基的单克隆抗体特异性抑制。类似地，IL-1处理导致β2整联蛋白介导的中性粒细胞对DMJ处理的内皮细胞的粘附以剂量依赖性方式增加。然而，IL-1诱导的粘附没有被抗ICAM-1抗体显著抑制，因此，表明在内皮细胞表面上存在其他诱导组分。我们的结果表明，N-连接寡糖糖基化的改变，导致可能与β2整联蛋白相互作用的分子上高甘露糖型糖的合成，导致PMA激活的中性粒细胞与内皮细胞的粘附增加。© 1993 Wiley利斯公司
We report herein on the role of N‐linked oligosaccharide processing of endothelial cell surface proteins on the adhesion of neutrophils. Monolayers of human umbilical vein endothelial cells were treated for 24 h with deoxymannojirimycin (DMJ), an inhibitor of golgi mannosidase I, which results in changes in glycoprotein processing, and then incubated with neutrophils to examine their ability to adhere to the treated endothelial cells. Treatment with DMJ, which leads to accumulation of high mannose type oligosaccharides, resulted in a twofold increase in adherence of phorbol ester (PMA) activated neutrophils compared to attachment to untreated endothelial cells. This adherence was likely mediated by the β2 integrin, Mac‐1, and could be specifically inhibited with monoclonal antibodies to ICAM‐1 and to the integrin β2 subunit. Similarly, IL‐1 treatment resulted in a β2 integrin mediated increase in neutrophil adherence to the DMJ treated endothelial cells in a dose dependent manner. However, the IL‐1 induced adherence was not significantly inhibited by the anti‐ICAM‐1 antibody, thus, suggesting the presence of other inducible components on the endothelial cell surface. Our results demonstrate that alterations in glycosylation of N‐linked oligosaccharides, resulting in the synthesis of high mannose type sugars on molecules that may interact with the β2 integrins, leads to an increased adherence of PMA activated neutrophils to endothelial cells. © 1993 Wiley‐Liss, Inc.