Depression of endothelium-dependent relaxation by filarial parasite products.
Depression of endothelium-dependent relaxation by filarial parasite products.
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丝虫寄生虫产物抑制内皮依赖性松弛。
DOI：
10.1152/ajpheart.1990.259.2.h648
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Williams,JF
中科院分区：
文献类型：
--
作者：
Kaiser,L;Tithof,PK;Williams,JF
心丝虫、免疫dirofilia、感染犬股动脉内皮依赖性松弛抑制。此外，在受感染的狗身上，放松的机制是不同的。由于D. immitis主要位于肺循环，这些发现表明D. immitis释放生物活性介质，改变远端内皮依赖性松弛。我们在体外测试了这个假设。将大鼠主动脉环暴露于D. immitis（单独，在1000 mol wt或100 mol wt的切断透析管中，并用条件培养基进行生物测定）。D.单独免疫抑制对乙酰胆碱、苯酚和A23187的松弛。硝酸甘油松弛不受影响。在1000 mol wt透析管和条件培养基中进行生物测定时，可以观察到单独使用D. immitis时乙酰胆碱松弛的抑制。然而，100 mol wt透析管中乙酰胆碱松弛与对照组无明显差异。看来，D. immitis释放一个小的，稳定的，生物活性因子，改变内皮依赖性松弛。它的确切性质尚不清楚。研究丝虫病中内皮细胞的行为以及丝虫病因子对内皮细胞功能的调节，可能为了解寄生虫病的发病机制提供重要线索。
Endothelium-dependent relaxation is depressed in the femoral artery of dogs with heartworm, Dirofilaria immitis, infection. Moreover, in infected dogs, the mechanism of relaxation is different. Because D. immitis is located primarily in the pulmonary circulation, these findings suggested that D. immitis releases biologically active mediators that alter distal endothelium-dependent relaxation. We tested this hypothesis in vitro. Rings of rat aorta were exposed to D. immitis (alone, in 1,000 mol wt or 100 mol wt cutoff dialysis tubing, and bioassay with conditioned medium). D. immitis alone depressed relaxation to acetylcholine, carbachol, and A23187. Nitroglycerin relaxation was not affected. Depression of acetylcholine relaxation was seen with D. immitis alone, in 1,000 mol wt dialysis tubing, and bioassay with conditioned medium. However, acetylcholine relaxation with D. immitis in 100 mol wt dialysis tubing was not different from control. It appears that D. immitis releases a small, stable, biologically active factor that alters endothelium-dependent relaxation. Its exact nature is unknown. The study of endothelial cell behavior in filariasis and modulation of endothelial cell function by filarial factors may provide important clues in understanding the pathogenesis of parasitic diseases.