Replication potentials of HIV-1/HSIV in PBMCs from northern pig-tailed macaque (Macaca leonina)
Replication potentials of HIV-1/HSIV in PBMCs from northern pig-tailed macaque (Macaca leonina)
复制标题
HIV-1/HSIV 在北方猪尾猕猴 (Macaca leonina) PBMC 中的复制潜力
DOI：
10.11813/j.issn.0254-5853.2014.3.186
复制
发表时间：
2014-05-01
影响因子：
4.9
通讯作者：
Zheng, Yong-Tang
中科院分区：
文献类型：
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作者：
Lei, Ai-Hua;Zhang, Gao-Hong;Zheng, Yong-Tang
北方猪尾猕猴（Macaca leonina）是一种独立的旧大陆猴种，我们以前发现，来自猕猴的PBMC对人类免疫缺陷病毒1型（HIV-1）易感，这可能是由于缺乏限制HIV-1复制的TRIMS蛋白。在这里，我们调查了6个实验室适应的HIV-1毒株和3个主要的HIV-1分离株在狮支原体PBMC中的感染潜力。结果表明，这些毒株的复制水平各不相同，但都很低，其中HIV-1（NL 4 -3）的复制能力最强。基于限制性因子APOBEC 3和HIV/SIV衍生的Vif蛋白之间存在物种特异性相互作用的大量证据，我们随后检查了Vif取代的HIV-1（HSIV）在M leonina PBMC中的复制潜力。值得注意的是，HSIV-vif（mac）和stHIV-1（sv）嵌合体，两种HIV-1（NL 4 -3）衍生的病毒编码SIVmac 239的病毒感染因子（Vif）蛋白，在M leonina细胞中稳健复制，这表明HSIV可以有效拮抗M leonina细胞中表达的APOBEC 3蛋白的抗病毒活性。因此，我们的数据表明，M leonina有潜力发展成为一个有前途的动物模型，人类艾滋病。
The northern pig-tailed macaque (Macaca leonina) has been identified as an independent species of Old World monkey, and we previously found that PBMCs from M leonina were susceptible to human immunodeficiency virus type 1 (HIV-1), which may be due to the absence of a TRIMS protein restricting HIV-1 replication. Here we investigated the infection potentials of six laboratory adapted HIV-1 strains and three primary HIV-1 isolates in PBMCs from M leonina. The results indicate that these strains are characterized by various but low replication levels, and among which, HIV-1(NL4-3) shows the highest replication ability. Based on the abundant evidence of species-specific interactions between restriction factors APOBEC3 and HIV/SIV-derived Vif protein, we subsequently examined the replication potentials of vif-substituted HIV-1 (HSIV) in M leonina PBMCs. Notably, HSIV-vif(mac) and stHIV-1(sv) chimeras, two HIV-1(NL4-3)-derived viruses encoding the viral infectivity factor (Vif) protein from SIVmac239, replicated robustly in cells from M leonina, which suggests that HSIV could effectively antagonize the antiviral activity of APOBEC3 proteins expressed in cells of M leonina. Therefore, our data demonstrate that M leonina has the potential to be developed into a promising animal model for human AIDS.