ACTIVATION OF CARDIAC VAGAL AFFERENTS IN ISCHEMIA AND REPERFUSION - PROSTAGLANDINS VERSUS OXYGEN-DERIVED FREE-RADICALS
ACTIVATION OF CARDIAC VAGAL AFFERENTS IN ISCHEMIA AND REPERFUSION - PROSTAGLANDINS VERSUS OXYGEN-DERIVED FREE-RADICALS
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DOI：
10.1161/01.res.74.5.904
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发表时间：
1994-05-01
影响因子：
20.1
通讯作者：
SCHULTZ, HD
中科院分区：
文献类型：
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作者：
USTINOVA, EE;SCHULTZ, HD
心肌缺血和再灌注可引起心脏迷走神经末梢兴奋和心源性降压反射激活（Bezold-Tarisch效应）。我们推测，氧衍生的自由基，这是已知的长时间缺血和再灌注过程中产生的，有助于这种传入兴奋。我们记录了31只大鼠的47条化学敏感迷走神经传入纤维的活动，这些纤维的末梢位于左心室。化学敏感的结束，确定与辣椒素（10微克）的心脏表面的局部应用。通过局部应用H2 O2（3至9 μ mol）来评估末端对氧自由基的反应性。在左冠状动脉前降支（LAD）闭塞30分钟和随后再灌注10分钟期间记录迷走神经纤维的活动。缺血区内化学敏感末梢的活性在前降支闭塞的前2分钟从每秒2.2+/-0.4增加到每秒4.3+/-0.9（增加107+/-30%，P
Myocardial ischemia and reperfusion can evoke excitation of cardiac vagal nerve endings and activation of a cardiogenic depressor reflex (Bezold-Tarisch effect). We postulate that oxygen-derived free radicals, which are known to be produced during prolonged ischemia and reperfusion, contribute to this afferent excitation. We recorded activity from 47 chemosensitive vagal afferent fibers in 31 rats; the endings of these fibers were located in the left ventricle. Chemosensitive endings were identified with topical applications of capsaicin (10 mu g) to the surface of the heart. Reactivity of the endings to oxygen-derived free radicals was assessed by topical application of H2O2 (3 to 9 mu mol). Activity of the vagal fibers was recorded during 30 minutes of occlusion of the left anterior descending coronary artery (LAD) and 10 minutes of subsequent reperfusion. The activity of chemosensitive endings within the ischemic zone increased in the first 2 minutes of LAD occlusion from 2.2+/-0.4 to 4.3+/-0.9 impulses per second (107+/-30% increase, P