Flash photolysis using a light emitting diode: An efficient, compact, and affordable solution
Flash photolysis using a light emitting diode: An efficient, compact, and affordable solution
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DOI：
10.1016/j.ceca.2005.03.001
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发表时间：
2005-06-01
期刊：
影响因子：
4
通讯作者：
Chatton, JY
中科院分区：
文献类型：
--
作者：
Bernardinelli, Y;Haeberli, C;Chatton, JY
闪光光解已成为活细胞和组织动态研究的基本技术。这种方法提供了几个优点，可以以高空间分辨率即时改变活细胞内外生物活性化合物的浓度。用于光解的光源需要在近UV范围（300-380 nm）内提供高强度光脉冲，以在短时间内光活化足够量的分子。通常需要紫外激光作为光源，这使得闪光光解成为一种昂贵的方法。在这里，我们描述了一个高功率365 nm的发光二极管（UV LED）耦合到一个光纤，以精确地将光传递到样品的使用。在神经元和星形胶质细胞中证明了UV LED光源光活化几种笼状化合物（CMNB-荧光素、MNI-谷氨酸盐、NP-EGTA、DMNPE-ATP）以及引起相关细胞Ca（2+）响应的能力。该报告表明，紫外LED是闪光光解的有效光源，并在许多应用中代表了紫外激光的替代品。一个紧凑，功能强大，低成本的系统进行了详细描述。(c)2005爱思唯尔有限公司保留所有权利。
Flash photolysis has become an essential technique for dynamic investigations of living cells and tissues. This approach offers several advantages for instantly changing the concentration of bioactive compounds outside and inside living cells with high spatial resolution. Light sources for photolysis need to deliver pulses of high intensity light in the near UV range (300-380 nm), to photoactivate a sufficient amount of molecules in a short time. UV lasers are often required as the light source, making flash photolysis a costly approach. Here we describe the use of a high power 365 mn light emitting diode (UV LED) coupled to an optical fiber to precisely deliver the light to the sample. The ability of the UV LED light source to photoactivate several caged compounds (CMNB-fluorescein, MNI-glutamate, NP-EGTA, DMNPE-ATP) as well as to evoke the associated cellular Ca(2+) responses is demonstrated in both neurons and astrocytes. This report shows that UV LEDs are an efficient light source for flash photolysis and represent an alternative to UV lasers for many applications. A compact, powerful, and low-cost system is described in detail. (c) 2005 Elsevier Ltd. All rights reserved.