The beginning of the calcium transient in rat embryonic heart
The beginning of the calcium transient in rat embryonic heart
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DOI：
10.1007/s12576-010-0131-x
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发表时间：
2011-03-01
影响因子：
2.3
通讯作者：
Tohse, Noritsugu
中科院分区：
文献类型：
--
作者：
Kobayashi, Takeshi;Maeda, Sachiko;Tohse, Noritsugu
虽然许多研究人员试图观察心跳的开始，但没有研究表明钙瞬变的开始。在这里，我们评估开始的钙瞬变在Wistar大鼠心脏。我们首先试图揭示Wistar大鼠的心脏何时开始收缩，因为以前没有研究评估过Wistar大鼠心跳的开始。将胚胎转移到安装在显微镜上的小型培养箱中观察发现，心脏原基，即所谓的心脏新月体，在胚胎第9.99-10.13天开始收缩。观察加载Fluo-3 AM的胚胎发现，钙瞬变的开始先于收缩的开始，收缩的开始先于线性心管的出现。硝苯地平（1 μ M），但不是ryanodine（1 μ M），取消钙瞬变。这些结果表明，钙瞬变在胚胎早期只涉及钙进入L-型钙通道的情况下，在成熟的心脏。这项研究首次证明了心脏原基的形态变化与钙瞬变和收缩开始之间的关系。
Although many researchers have tried to observe the beginning of the heartbeat, no study has shown the beginning of the calcium transient. Here, we evaluate the beginning of the calcium transient in the Wistar rat heart. We first tried to reveal when the heart of the Wistar rat begins to contract because no previous study has evaluated the beginning of the heartbeat in Wistar rats. Observation of embryos transferred to a small incubator mounted on a microscope revealed that the heart primordium, the so-called cardiac crescent, began to contract at embryonic day 9.99-10.13. Observation of embryos loaded with fluo-3 AM revealed that the beginning of the calcium transient precedes the initiation of contraction which precedes the appearance of the linear heart tube. Nifedipine (1 mu M), but not ryanodine (1 mu M), abolished the calcium transients. These results indicate that calcium transients in the early embryonic period involve exclusively calcium entry through L-type calcium channels in contrast to the situation in mature hearts. This study provides the first demonstration of the relationship between morphological changes in the heart primordium and the beginning of the calcium transient and contraction.