Antimicrobial resistance profiles of Shiga toxin-producing Escherichia coli O157 and Non-O157 recovered from domestic farm animals in rural communities in Northwestern Mexico.
Antimicrobial resistance profiles of Shiga toxin-producing Escherichia coli O157 and Non-O157 recovered from domestic farm animals in rural communities in Northwestern Mexico.
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DOI：
10.1186/s13756-015-0100-5
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发表时间：
2016
影响因子：
5.5
通讯作者：
Chaidez C
中科院分区：
文献类型：
--
作者：
Amézquita-López BA;Quiñones B;Soto-Beltrán M;Lee BG;Yambao JC;Lugo-Melchor OY;Chaidez C
产志贺毒素大肠杆菌（STEC） O157和非O157的抗微生物药物耐药性日益受到关注。本研究的目的是调查从墨西哥西北部库利亚坎河谷农业农场动物粪便中回收的产志在大肠杆菌O157和非O157的耐药谱。所有检测的产志贺毒素大肠杆菌菌株均对头孢他啶、头孢曲松、环丙沙星、萘啶酸和甲氧苄啶-磺胺甲恶唑五种抗菌素敏感。然而，对氨苄西林、头孢菌素、氯霉素和卡那霉素这四种抗菌素的耐药性普遍存在。有趣的是，在所检测的产志在大肠杆菌菌株中，对头孢菌素不敏感的菌株占主导地位，对应于85%（22/26）来自牛、羊和鸡的O157:H7菌株和73%（24/33）来自牛和羊的非O157菌株。统计分析显示，38%（10/26）来自多种动物来源的产志在大肠杆菌O157:H7菌株对氨苄西林的耐药性显著相关。在血清型、来源和抗菌素耐药性之间发现了另一个显著的相关性；从绵羊身上回收的所有O20:H4菌株都对四环素具有高度耐药性。42%（22/53）临床相关血清型为O8:H9、O75:H8、O146:H21和O157:H7的非敏感产志在大肠杆菌菌株中发现多药耐药。产大肠杆菌O157和非O157菌株来自Culiacan山谷的家畜，对墨西哥常用的氨基糖苷类、四环素类、头孢菌素和青霉素等抗菌素具有耐药性，但对氟喹诺酮类药物、喹诺酮类药物和磺胺类药物敏感。这些发现提供了基本信息，将有助于在墨西哥西北部一个重要农业区监测抗菌素耐药性。本文的在线版本（doi:10.1186/s13756-015-0100-5）包含补充材料，可供授权用户使用。
Antimicrobial resistance in Shiga toxin-producing Escherichia coli (STEC) O157 and non-O157 is a matter of increasing concern. The aim of the present study was to investigate the antimicrobial resistance profiles of STEC O157 and non-O157 recovered from feces of domestic farm animals in the agricultural Culiacan Valley in Northwestern Mexico. All of the examined STEC strains showed susceptibility to five antimicrobials, ceftazidime, ceftriaxone, ciprofloxacin, nalidixic acid, and trimethoprim-sulfamethoxazole. However, resistance to the four antimicrobials, ampicillin, cephalothin, chloramphenicol, and kanamycin was commonly observed. Interestingly, non-susceptibility to cephalothin was predominant among the examined STEC strains, corresponding to 85 % (22/26) of the O157:H7 from cattle, sheep and chicken and 73 % (24/33) of the non-O157 strains from cattle and sheep. Statistical analyses revealed that resistance to ampicillin was significantly correlated to 38 % (10/26) of STEC O157:H7 strains from multiple animal sources. Another significant correlation was found between serotype, source, and antimicrobial resistance; all of the O20:H4 strains, recovered from sheep, were highly resistant to tetracycline. Multidrug resistance profiles were identified in 42 % (22/53) of the non-susceptible STEC strains with clinically-relevant serotypes O8:H9, O75:H8, O146:H21, and O157:H7. STEC O157 and non-O157 strains, recovered from domestic farm animals in the Culiacan Valley, exhibited resistance to classes of antimicrobials commonly used in Mexico, such as aminoglycosides, tetracyclines, cephalosporins and penicillin but were susceptible to fluoroquinolones, quinolones, and sulfonamides. These findings provide fundamental information that would aid in the surveillance of antimicrobial resistance in an important agricultural region in Northwestern Mexico. The online version of this article (doi:10.1186/s13756-015-0100-5) contains supplementary material, which is available to authorized users.