Identification and Characterization of the WOX Family Genes in Five Solanaceae Species Reveal Their Conserved Roles in Peptide Signaling
Identification and Characterization of the WOX Family Genes in Five Solanaceae Species Reveal Their Conserved Roles in Peptide Signaling
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五种茄科物种 WOX 家族基因的鉴定和表征揭示了它们在肽信号转导中的保守作用。
DOI：
10.3390/genes9050260
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发表时间：
2018-05-01
期刊：
影响因子：
3.5
通讯作者：
Guo, Yongfeng
中科院分区：
文献类型：
--
作者：
Li, Xiaoxu;Hamyat, Madiha;Guo, Yongfeng
植物特异性WOX（WUSCHEL相关同源框）转录因子家族成员在多种发育过程中参与调控干细胞维持和细胞命运的肽信号转导。尽管拟南芥WOX基因的研究已经取得了显著的进展，但对茄科物种中WOX基因的研究却知之甚少。从5种茄科植物中共鉴定出45个WOX成员，其中马铃薯8个，绒毛烟草8个，番茄10个，欧洲烟草10个，烟草9个。利用3种不同的方法进行系统发育分析，将新鉴定的WOX成员分为3个分支和9个亚群。WOX蛋白的外显子-内含子结构和基序组织模式与系统发育结果一致。基因重复事件和正在进行的进化揭示了系统发育树上的额外分支和部分WUS盒的存在下，在一些非WUS分支成员。基因表达与或没有CLE（clavata 3（clv 3）/胚胎周围区域相关）肽处理显示，烟草WOX基因表现出相似或不同的表达模式相比，其拟南芥同源物，这表明功能保守或分歧。烟草WUSCHEL（NtabWUS）在组织中心的表达可以拯救拟南芥中的wus-1突变体表型，这意味着茄科WOX蛋白在肽介导的植物发育调控中的保守作用。
Members of the plant-specific WOX (WUSCHEL-related homeobox) transcription factor family have been reported to play important roles in peptide signaling that regulates stem cell maintenance and cell fate specification in various developmental processes. Even though remarkable advances have been made in studying WOX genes in Arabidopsis, little is known about this family in Solanaceae species. A total of 45 WOX members from five Solanaceae species were identified, including eight members from Solanum tuberosum, eight from Nicotiana tomentosiformis, 10 from Solanum lycopersicum, 10 from Nicotiana sylvestris and nine from Nicotiana tabacum. The newly identified WOX members were classified into three clades and nine subgroups based on phylogenetic analysis using three different methods. The patterns of exon-intron structure and motif organization of the WOX proteins agreed with the phylogenetic results. Gene duplication events and ongoing evolution were revealed by additional branches on the phylogenetic tree and the presence of a partial WUS-box in some non-WUS clade members. Gene expression with or without CLE (clavata3 (clv3)/embryo surrounding region-related) peptide treatments revealed that tobacco WOX genes showed similar or distinct expression patterns compared with their Arabidopsis homologues, suggesting either functional conservation or divergence. Expression of Nicotiana tabacum WUSCHEL (NtabWUS) in the organizing center could rescue the wus-1 mutant phenotypes in Arabidopsis, implying conserved roles of the Solanaceae WOX proteins in peptide-mediated regulation of plant development.