The extracellular matrix protein Del1 induces apoptosis via its epidermal growth factor motif
The extracellular matrix protein Del1 induces apoptosis via its epidermal growth factor motif
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DOI：
10.1016/j.bbrc.2010.02.076
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发表时间：
2010-03-19
影响因子：
3.1
通讯作者：
Hidai, Chiaki
中科院分区：
文献类型：
--
作者：
Kitano, Hisataka;Kokubun, Shinichiro;Hidai, Chiaki
小鼠Del 1是一种细胞外基质蛋白，主要在发育中的胚胎中表达。Del 1有三个EGF基序和两个盘状结构域。据报道，第二个EGF基序含有与整联蛋白受体结合的RGD序列。在这里，我们提供的证据表明，Del 1蛋白在体外诱导细胞死亡。染色质浓缩和DNA梯状条带，提示细胞凋亡。TUNEL法和Annexin V染色分析结果也与细胞凋亡一致。Del 1的促凋亡活性可定位于第三个EGF基序，其符合共有序列CX（D/N）<$（F/Y）XCXC，其中天冬氨酸残基（D）可被β-羟基化。25皮摩尔的重组E3就能诱导细胞凋亡。(C)2010年爱思唯尔公司All rights reserved.
Mouse Del1 is an extracellular matrix protein mainly expressed in the developing embryo. Del1 has three EGF motifs and two discoidin domains. The second EGF motif reportedly contains an RGD sequence that binds to integrin receptors. Here, we provide evidence that Del1 protein induces cell death in vitro. Chromatin condensation and DNA laddering were observed, suggestive of apoptosis. The results of analysis using the TUNEL method and annexin V staining were also consistent with apoptosis. The apoptosis-inducing activity of Del1 could be mapped to the third EGF motif, which fitted the consensus sequence CX(D/N)XXXX(F/Y)XCXC, wherein the aspartic acid residue (D) could be beta-hydroxylated. As little as twenty-five picomolar of recombinant E3 could induce apoptosis. (C) 2010 Elsevier Inc. All rights reserved.